Calmodulin (CaM, CALM1, CALM, CALM2, CAMB, CALM3, CALML2, CAM3, CAMC, CAMIII)

Calmodulin, a ubiquitous eukaryotic calcium binding protein, is a principal mediator of the calcium signal. It participates in signalling pathways inducing proliferation, motility and cell cycle progression. Human calmodulin is encoded by three genes CALM1, CALM2 and CALM3 located on different chromosomes. The vertebrate CALM family of genes is unique in that its members specify an identical protein. The protein itself is made up of 148aa and has four calcium binding domains. As calmodulin is essential for normal cell function, it is likely that levels are tightly controlled both temporally and spatially. Immunohistochemical staining for calmodulin has been reported in the epithelia of testis, breast, stomach, prostate, gall bladder as well as in macrophages, fibroblasts and sebaceous glands within the dermis of skin. In healing skin wounds, calmodulin is found at its highest levels in maturing keratinocytes. It is noticeably abundant in epidermis close to a wound and re-epithelializing margins where calcium levels are highest. In studies of Alzheimer's brains, calmodulin immunostaining has been reported to be lost in cortical regions where large amounts of aluminium have accumulated.\n\nApplications:\nSuitable for use in Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nImmunohistochemistry (Formalin fixed paraffin embedded): 1:25-1:50 (High temperature antigen unmasking technique. 60min primary antibody incubation at 25 degrees C. Standard ABC technique.)\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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