CART (Cocaine and Amphetamine Related Transcript, CARP)

Cocaine and Amphetamine Related Transcript (CART) was initially identified using PCR differential display as mRNA whose levels in the brain was specifically induced by psychomotor stimulants such as cocaine and amphetamine. CART encodes a secretory single chain polypeptide of 129aa or 116aa protein due to the usage of alternate splicing and processing. The first 27aa represents the hydrophobic core indicative of signal peptide. The CART protein also has several basic amino acids near the 54-66 residues with a potential to form multiple proteolytic forms. CART mRNA was specially enriched in the hypothalamus. In the striatum, CART expression was induced 4-5 fold by cocaine and amphetamine. CART is highly conserved between human and mouse (95% amino acid homology); CART gene was localized to human chr 5.\nCART has been found to control satiety modulating the actions of two key regulators of food intake, leptin and NPY. Starvation decreases CART levels in the arcuate nucleus (3). Obese animals have virtually no CART. CART inhibits both normal and starvation-induced feeding, and completely inhibits NPY-induced feeding. Immunoneutralization of CART by administration of anti-CART resulted into higher food intake suggesting that CART is an endogenous regulator of food intake. \n\nApplications:\nSuitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications not tested. \n\nRecommended Dilutions:\nWestern Blot: 1-10ug/ml using ECL.\nELISA: 1:10,000-1:50,000 using 50-100ng control peptide/well\nImmunohistochemistry: 5-10ug/ml\nOptimal dilutions to be determined by the researcher.\n\nRecommended Control Peptide: \nC1386-50A: CART, aa55-102 Control Peptide \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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