CART (Cocaine and Amphetamine Related Transcript, CARP)\n

Cocaine- and Amphetamine Related Transcript (CART) was initially identified using PCR differential display as mRNA whose levels in the brain was specifically induced by psychomotor stimulants such as cocaine and amphetamine. CART encodes a secretory single chain polypeptide of 129aa or 116aa protein due to the usage of alternate splicing and processing. The first 27aa represents the hydrophobic core indicative of signal peptide. The CART protein also has several basic amino acids near the 54-66 residues with a potential to form multiple proteolytic forms. CART mRNA was specially enriched in the hypothalamus. In the striatum, CART expression was induced 4-5 fold by cocaine and amphetamine. CART is highly conserved between human and mouse (95% amino acid homology); CART gene was localized to human chromosome Most recently, CART has been found to control satiety modulating the actions of two key regulators of food intake, leptin and NPY. Starvation decreases CART levels in the arcuate nucleus. Obese animals have virtually no CART. Peripheral administration of leptin in obese animals induces CART. Intracereberoventricular injection of recombinant CART inhibits both normal and starvation-induce feeding, and completely inhibits NPY-induce feeding. Immunoneutralization of CART by administration of anti-CART resulted into higher food intake suggesting that CART is an endogenous regulator of food intake. CART 55-102 (numbers corresponds to the predicted signal-peptide-cleavage site in the long form of CART) was physiologically active in inhibiting feeding in normal rats.\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000-1:5000\nELISA: 1:10,000-1:50,000, use 5-10ug control peptide/1ul\nOptimal dilutions to be determined by the researcher.\n\nControl Peptide: C1386-50A\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C1386-50D

Size

50ul

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Mouse

Form

Supplied as a liquid in PBS, 0.05% sodium azide, 40% glycerol.

P Type

Pab

Purity

Serum

Isotype

IgG

References

(1). Douglass J et al (1995) J Neurosci. 15, 2471; (2). Douglass J and Daoud S (1996) Gene 169, 241; (3). Kristensen P et al (1998) Nature 393, 72.\n\n

Additional Info

100% conserved in mouse, rat, sheep and 97% in human CART.