Catenin, beta, phosphorylated (Ser37) (catenin (cadherin-associated protein), beta 1, 88kD, beta-catenin, Beta-catenin, Catenin beta-1, CTNNB, DKFZp686D02253, FLJ25606, FLJ37923, OK/SW-cl.35, PRO2286)

Catenins are proteins found in complexes with cadherin cell adhesion molecules of animal cells. The first two catenins that were identified became known as alpha-catenin and beta-catenin. Alpha-catenin can bind to b-catenin and can also bind actin. Beta-catenin binds the cytoplasmic domain of some cadherins. When B-catenin was sequenced it was found to be a member of the armadillo family of proteins. These proteins have multiple copies of the so-called armadillo repeat domain which is specialized for protein-protein binding. An increase in B-catenin production has been noted in those people who have Basal Cell Carcinoma and leads to the increase in proliferation of related tumors. When B-catenin is not associated with cadherins and a-catenin, it can interact with other proteins such as Catenin Beta Interacting Protein 1 (ICAT) and adenomatosis polyposis coli (APC). Recent evidence suggests that B-catenin plays an important role in various aspects of liver biology including liver development (both embryonic and postnatal), liver regeneration following partial hepatectomy, HGF-induced hepatpomegaly, liver zonation, and pathogenesis of liver cancer. After phosphorylation, beta catenin is rapidly degraded into various fragments over time (1).\n\nApplications:\nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:500-1:1000 detects phosph-beta catenin in Calyculin/Okadoic acid treated and untreated HEK293 cell lysate.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

C2069-50P

Size

100ul

Applications

WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.

P Type

Pab

Purity

Purified by affinity chromatography.

Isotype

IgG

References

1. Giuseppina T., et al. (1998). J Biol Chem. 273, 51:33909-33914 .

Additional Info

Recognizes human beta Catenin phosphorylated at Ser37. Depending on the type of treatment and the amount of time elapsed after the phosphorylation event, will detect both full length beta Catenin phosphorylated at Ser37, as well as the degraded, fragmented form that results immediately after phosphorylation (1). Species Sequence Homology: mouse, rat, xenopus, chicken.