Catenin, beta, phosphorylated (Tyr142) (Beta Catenin, Cadherin-associated Protein, Catenin beta 1, CATNB, CTNNB, CTNNB1)

b-Catenin is a 92kD protein that binds to the cytoplasmic tail of E-Cadherin. The cadherins, transmembrane adhesion molecules, are found with catenins at adherens junctions. Deletions in the cytoplasmic domain of E-Cadherin eliminate catenin binding and result in a loss of cell adhesion. Tyrosine phosphorylation of b-Catenin can regulate its interaction with critical components of adherens junctions. Both Fer and Fyn kinases phosphorylate tyrosine 142 in vitro. Overexpression of these kinases in epithelial cells disrupts interactions between a- and b-Catenins. The phosphorylation of tyrosine 142 may act as a switch from the transcriptional to the adhesive role of b-Catenin. Src family kinases can also phosphorylate tyrosine 654 in the C-terminal armadillo repeat of b-Catenin. This phosphorylation regulates b-Catenin binding to E-cadherin. Thus, site-specific tyrosine phosphorylation of b-Catenin may regulate specific protein-protein interactions leading to changes in cell adhesion.\n\nApplications:\nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:500\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nHct116 src transformed cells treated with pervanadate. Human endothelial, A431, and HeLa cells may also be used.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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