Cav1.2 (Voltage-dependent L-type calcium channel subunit alpha-1C, Voltage-gated calcium channel subunit alpha Cav1.2, CACNA1C, Calcium channel L type, alpha-1 polypeptide isoform 1 cardiac muscle )

Ion channels are integral membrane proteins that help establish and control the small voltage gradient across the plasma membrane of living cells by allowing the flow of ions down their electrochemical gradient. Where some ion channels permit the passage of ions based on charge, others conduct based on an ionic species, such as sodium or potassium. Furthermore, in some ion channels, the passage is governed by a gate which is controlled by chemical or electrical signals, temperature or mechanical forces. There are a few main classifications of gated ion channels. The first are voltage-gated ion channels which open and close in response to membrane potential. These are then separated into sodium, calcium, potassium, proton, transient receptor and cyclic nucleotide-gated channels. Ligand-gated ion channels open in response to specific ligand molecules binding to the extracellular domain of the receptor protein. The other gated classifications include activation and inactivation by second messengers, inward-rectifier potassium channels, calcium-activated potassium channels, two-pore-domain potassium channels, light-gated channels, mechano-sensitive ion channels and cyclic nucleotide-gated channels. Finally, the other classifications are based on less normal characteristics such as two-pore channels, and transient receptor potential channels. Specifially, Cav1.2 is a cardiac L-type calcium channel, and is important for excitation and contraction of the heart. It may be associated with a variant of Long QT syndrome called Timothy's syndrome and also with Brugada syndrome. Some references also suggest it is related to bipolar disease as well. \n\nApplications:\nSuitable for use in Western Blot, Immunoprecipitation, Immunofluorescence and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1ug/ml. If results are poor, use lysate without boiling; heat at 37 degrees C for 15 minutes.\nImmunohistochemistry: 0.1-1ug/ml using peroxidase method\nImmunohistochemistry: 1-10ug/ml using Immunofluorescence.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

C2097-85B4

Size

100ug

Applications

IHC, IP, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide, 50% glycerol.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG2b

References

1. Hille B. (2001) Ion Channels of Excitable Membranes, 3rd Ed., Sinauer Associated Inc.:Sunderland, MA USA. 2. www.iochannels.org 3. Splawski I., et al. (2004) Cell. 119 (1): 19-31. 4. Krey J.F., and Dolmetsch R. (2009) Biophysical. 96 (3):221a-222a. 5. Crotti L., Celano G., Dagradi F. and Schwartz P.J. (2008) \nOrphanet J Rare Disease 3:18.

Additional Info

Recognizes Cav1.2 at ~240kD. Species Crossreactivity: human, rat and mouse