CCR4 (CC chemokine receptor type 4, CCCKR4, CCR4, ChemR13, CKR4, CMKBR4, HGCN 14099, K5 5, MGC88293)

Chemokine receptors are specialized proteins that function at the surface of cells, and enable chemokines to trigger internal cellular processes. Chemokines and their receptors play a pivotal role in lymphocyte trafficking, recruiting and recirculation. Chemokines act through seven transmembrane G protein-coupled receptors. The chemokine system is increasingly recognized to play an important role in lymphocyte biology.\n\nCCR4 is a chemokine receptor and is preferentially expressed on type 2 helper T (Th2-type) cells. In contrast to other chemokine receptors, the expression of CCR4 and CCR8 on Th2 cells is transiently increased following TCR and CD28 engagement. Moreover, activated Th1 cells up-regulate CCR4 expression and functional responsiveness to thymus- and activation-regulated chemokine.\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested. \n\nRecommended Dilution:\nWestern Blot: 1-2ug/ml In Daudi cell lysate, a band at 42kD should be detected.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C2099-93

Size

100ug

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.

P Type

Pab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG

References

1. Murphy, P. M.. 1996. Cytokine Growth Factor Rev. 7: 47. 2. Neote, K., et al., Cell 72: 415. 3. Gao, J. L., et al.,. J. Biol. Chem. 270: 17494 4. Gao, J. L., et al., Biochem. Biophys. Res. Commun. 223: 679.

Additional Info

Recognizes human CCR4. Cross reacts with mouse and rat CCR4.