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CCR4 (CC chemokine receptor type 4, CCCKR4, CCR4, ChemR13, CKR4, CMKBR4, HGCN 14099, K5 5, MGC88293)

Cat no: C2099-93E

CCR4 (CC chemokine receptor type 4, CCCKR4, CCR4, ChemR13, CKR4, CMKBR4, HGCN 14099, K5 5, MGC88293)

Chemokine receptors are specialized proteins that function at the surface of cells, and enable chemokines to trigger internal cellular processes. Chemokines and their receptors play a pivotal role in lymphocyte trafficking, recruiting and recirculation. Chemokines act through seven transmembrane G protein-coupled receptors. The chemokine system is increasingly recognized to play an important role in lymphocyte biology. CCR4 is a chemokine receptor and is preferentially expressed on type 2 helper T (Th2-type) cells. In contrast to other chemokine receptors, the expression of CCR4 and CCR8 on Th2 cells is transiently increased following TCR and CD28 engagement. Moreover, activated Th1 cells up-regulate CCR4 expression and functional responsiveness to thymus- and activation-regulated chemokine.\n\nApplications:\nSuitable for use in Flow Cytometry, Western Blot and Immunohistochemistry. Other applications not tested. \n\nRecommended Dilutions: \nFlow Cytometry: 1-3ul per 2x10e5 cells per 50ul\nWestern Blot: 1:1000\nOptimal dilution determined by the researcher. \n\nPositive Control:\nMouse spleen cells\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

C2099-93E

Size

200ug

Applications

FC, IHC, WB

Hosts

Goat

Reactivities

Mouse

Form

Supplied as a liquid in PBS, 1mg/ml BSA, 0.1% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

F(ab

References

Molecular cloning of murine CC CKR-4 and high affinity binding of chemokines to murine and human CC CKR-4: A. Hoogewerf, et al.; BBRC 218, 337 (1996)

Additional Info

Recognizes mouse CCR4. Does not crossreact with human CCR4.

Read more on Supplier website

Applications

ELISA

Reactivities

Hum

More info

Applications

IF

Hosts

Mouse

More info

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, IHC, WB

Hosts

Mouse

More info

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum

More info

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

More info
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