Home  >  Products  >  CCR4-NOT transcription complex subunit 4 (CNOT4, CCR4-associated factor 4, CLONE243, E3 ubiquitin protein ligase CNOT4, NOT4, NOT4H, Potential transcriptional repressor NOT4Hp)
CCR4-NOT transcription complex subunit 4 (CNOT4, CCR4-associated factor 4, CLONE243, E3 ubiquitin protein ligase CNOT4, NOT4, NOT4H, Potential transcriptional repressor NOT4Hp)

CCR4-NOT transcription complex subunit 4 (CNOT4, CCR4-associated factor 4, CLONE243, E3 ubiquitin protein ligase CNOT4, NOT4, NOT4H, Potential transcriptional repressor NOT4Hp)

Cat no: C2099-68W6

Supplier: United States Biological
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CNOT4 is a component of the CCR4-NOT transcription complex, a complex that is implicated in the repression of RNA polymerase II transcription. In the CCR4-NOT complex, CNOT4 acts as an E3 ubiquitin-protein ligase and interacts with a subset of E2 ubiquitin-conjugating enzymes through a unique C4C4 RING domain. This E3 ligase activity was shown to be dependent on the selective and specific interaction with the ubiquitin conjugating enzyme UbcH5B. In yeast, mutations in CNOT4 that prevented its interaction with the UbcH5B homolog UBC4 caused increased sensitivity to hydroxyurea, heat shock, and hygromycin B, suggesting that CNOT4 and UbcH5B are involved in stress response in vivo. Multiple isoforms of CNOT4 are known to exist. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1-2ug/ml Optimal dilutions to be determined by the researcher. Positive Control: A549 Cell Lysate Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: C2099-68W6
Reactivities: Human, Mouse, Rat
Hosts: Rabbit
Applications: Western Blot
Size: 100ug
Form: Supplied as a liquid in PBS, 0.02% sodium azide.
P type: Pab
Purity: Purified by immunoaffinity chromatography.
References: 1. Albert TK, Hanzawa H, Legtenberg IA, et al. Identification of a ubiquitin-protein ligase subunit within the CCR4-NOT transcription repressor complex. EMBO J. 2002; 21:355-64. 2. Winkler GS, Albert TK, Dominguez C, et al. An altered specificity ubiquitin-conjugating enzyme/ubiquitin-protein ligase pair. J. Mol. Biol. 2004; 337:157-65. 3. Mulder KW, Inagaki A, Cameroni E, et al. Modulation of Ubc4p/Ubc5p-mediated stress responses by the RING-finger-dependent ubiquitin-protein ligase Not4p in Saccharomyces cerevisiae. Genetics 2007; 176:181-92.
Additional info: Recognizes human CNOT4. Species Crossreactivity: Mouse and rat.

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