CCR5 (NT) (Chemokine Receptor 5, CC-CKR-5, CD195)

Human immunodeficiency virus (HIV) and related virus require coreceptors, in addition to CD4, to infect target cells. Some G protein-coupled receptors including CCR5, CXCR4, CCR3, CCR2b and CCR8 in the chemokine receptor family, and four new human molecules GPR15, STRL33, GPR1 and V28 were recently identified as HIV coreceptors. Among them, CCR5 (CC-CKR-5) is a principal coreceptor for macrophage- and dual-tropic HIV-1 strains fusion and entry of human white blood cells. CCR5 is required for the infection by HIV-1, HIV-2, and SIV. The b-chemokines RANTES, MIP-1a and MIP-1b are the ligands for CCR5 and prevent infection by M-tropic HIV-1. CXC5 associates with the surface CD4-gp120 of HIV complex and leads to membrane fusion and virus entry of target cells. The amino-terminal domain and the extracellular loops of CCR5 serve as HIV biding sites. CCR5 messenger RNA is expressed in lymphoid organs and monocytes. \n\nApplications: \nSuitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000-1:2000\nImmunohistochemistry: 20ug/ml\nOptimal dilutions to be determined by the researcher.\n\nWB Positive Control: \nTHP-1 whole cell lysate\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

C2099-99

Size

100ug

Applications

ELISA, IHC, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, 0.02% sodium azide.

P Type

Pab

Purity

Purified by DEAE chromatography.

Isotype

IgG

References

1. Dimitrov, DS. Cell 1997;91:721-30 2. Littman, DR. Cell 1998;93:677-80 3. Deng, H, et al. Nature 1996;381:661-6 4. Dragic, T, et al. Nature 1996;381:667-73 5. Coochi, F. et al. Science 1995;270:1811-5. 6. Wu, L. et al. Nature 1996;384:179-83 7. Trkola , A. et al. Nature 1996;384:184-7 8. Doranz, BJ. et al. J Virol 1997;71:6305-14 9. Dragic, T, et al. J Virol 1998;72:279-85 10. Raport, CJ, et al. J Biol Chem 1996;271:17161-6 11. Combadiere, C. et al. J Leuk Biol 1996;60:147-52

Additional Info

Recognizes human CCR5 at ~40kD.