Applications:
Suitable for use in ELISA, Flow Cytometry and Immunohistochemistry. Other applications not tested.
Recommended Dilution:
Flow Cytometry: Thymus cells, B?cells subset and HD?Mar cell line.
Immunohistochemistry: Cortical thymocytes (strong), Langerhans cells, interdigitating cells. Optimal dilutions to be determined by the researcher.
Hybridoma:
653 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.