CD10 (CALLA, CD 10, Common Acute Lymphocytic Leukemia Antigen, Enkephalinase, gp100, Membrane Metalloendopeptidase, MME, Neprilysin, Neutral Endopeptidase, Pmel17)

The cluster designation (CD) antigens are cell surface molecules first defined on human leukocytes and later found to be expressed by a variety of human cell types in both normal and pathologic states.The common acute lymphoblastic leukemia antigen (CALLA/CD10) is asingle- pass type II transmembrane metallo-endopeptidase that cleaves and inactivates a variety of peptide growth factors important for signal transduction including the enkephalins, bombesin, and substance P (1).It is expressed in a wide variety of epithelial and non-epithelial tissues, but its most significant application is in the diagnosis and classification of certain types of malignant lymphoma and leukemia. CD10 is expressed in a high percentage of cases of acute \nlymphoblastic leukemia, follicular lymphoma, Burkitt's lymphoma, and some hematopoietic tumors (2). It is also known to be a marker of endometrial stromal cells, and was reported to be expressed in cells immediately surrounding the neoplastic glands invading the myometrium (3). \n \nApplications:\nSuitable for use in Western Blot and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:5000-1:10000 \nImmunocytochemistry: 1:100\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.\n\nManufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.

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SPECIFICATIONS

Catalog Number

C2261-40S

Size

100ul

Applications

ICC, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in 50mM Tris-Glycine (pH 7.4), 0.15M sodium chloride, 40% glycerol, 0.01% sodium azide and 0.05% BSA.

P Type

Mab

Purity

Supernatant

Isotype

IgG

References

1. Dall'Era M A, et al. BMC Urology 7:3, 2007 2. McIntosh, G., et al. American Journal of Pathology 154:77-82, 1999 3. Ohishi Y, et al. Hum Pathol. 39(10):1446-53, 2008

Additional Info

Recognizes human CD10.

Alternative Names

CALLA, CD 10, Common acute lymphocytic leukemia antigen, Enkephalinase, gp100, Membrane metalloendopeptidase, MME, Neprilysin, Neutral endopeptidase, Pmel17