CD10 (CALLA, Common Acute Lymphocytic Leukemia Antigen, Neprilysin, NEP, Neutral Endopeptidase 24.11, EC3.4.24.11)

CD10 (CALLA, Common Acute Lymphocytic Leukemia Antigen, Neprilysin, NEP, Neutral Endopeptidase 24.11, EC3.4.24.11) is a single chain 100kD glycoprotein. It is a cell surface enzyme with neutral metalloendopeptidase activity which inactivates a variety of biologically active peptides. CD10 is expressed on the cells of lymphoblastic, BurkittпїЅs, and follicular germinal center lymphomas, and on cells from patients with chronic myelocytic leukemia (CML). It is also expressed on the surface of normal early lymphoid progenitor cells, immature B cells within adult bone marrow and germinal center B cells within lymphoid tissue. CD10 is also present on breast myoepithelial cells, bile canaliculi, fibroblasts, with especially high expression on the brush border of kidney and gut epithelial cells. \nAntigen Distribution: Granulocytes > 95%; cALL > 95%; Peripheral blood lymphocytes < 1%; T cells (lg+) < 1%; B cells (lg-) < 1%; Monocytes < 1%; Thymocytes < 1%; NK cells (CD16+) < 1%.\n\nApplications: \nSuitable for use in Immunofluorescence, Flow Cytometry and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nImmunohistochemistry: Acetone-fixed frozen\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nTonsil or small intestine\n\nStorage and Stability\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

C2261-49

Size

500ul

Applications

FC, IF, IHC

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

1. Guerin, S., et al. Cellular Immunology 175(1): 85-91 (1997). 2. Guerin, S., et al., Faseb Journal 11(5): 376-381 (1997). 3. Ishimaru, F., et al., Exp. Hematol. 24: 43-48 (1996). 4. Sato, Y., et al., Journal of Gastroenterology 31(1): 12-17 (1996). 5. Cirone, M., et al., Cancer Letters 89(1): 125-128 (1995). 6. de Saint-Vis, B., et al., Blood 86(3): 1098-1105 (1995). 7. Haouas, H., et al., Biochem. Biophys. Res. Comm. 207(3): 933-942 (1995). 8. Inukai, T., et al., Brit. J. Haematol. 89(3): 623-626 (1995). 9. Lapadula, G., et al., Clin. Exp. Rheumatol. 13(2): 143-148 (1995). 10. Benayahu, D., et al., J. Cell. Biochem. 56(1): 62-73 (1994). 11. Delikat, S.E., et al., Brit. J. Haematol. 87(3): 655-657 (1994). 12. Ganju, R.K., et al., J. Clin. Invest. 94: 1784-1791 (1994). 13. Mari, B., et al., Immunology 82(3): 433-438 (1994). 14. Pulczynski, S., Leukemia and Lymphoma 15(3-4): 243-252 (1994). 15. Carrel, S., et al., Melanoma Res. 3(5): 319-323 (1993). 16. Correale, P., et al., Year in Immunology 7: 90-95 (1993). 17. Penchansky, L., et al., Modern Path. 6(4): 414-418 (1993). 18. Pui, C.H., et al., Leukemia 7: 35-40 (1993). 19. Pulczynski, S., et al., Blood 78(7): 1834-1841 (1991). 20. Salles, G., et al., PNAS USA 90(16): 7618-7622 (1993). 21. Sandhaus, L.M., et al., Am. J. Ped. Hematology/Oncology 15(1): 65-70 (1993). 22. Imai, K., et al., Biol. Reprod. 46(3): 328-334 (1992). 23. Kee, B.L., et al., Intl. Immunol. 4(9): 1041-1047 (1992). 24. Milhiet, P.E., et al., Anal. Biochem. 205(1): 57-64 (1992).

Additional Info

Recognizes human CD10 at 100kD.