CD10 (Neprilysin, Atriopeptidase, Common Acute Lymphocytic Leukemia Antigen, CALLA, Enkephalinase, Neutral Endopeptidase 24.11, NEP, Neutral Endopeptidase, Skin Fibroblast Elastasem, SFE, MME, EPN)

CD10 is a 90-110kD cell surface glycoprotein expressed on normal early lymphoid progenitor cells, immature B-cells in adult bone marrow and mature germinal center B cells in lymphoid tissue. It is also expressed in some non-lymphoid tissues such as fibroblasts, breast myoepithelium, gut epithelial cells and brush border of Kidney. CD10 is a zinc-binding enzyme with a neutral metalloendopeptidase activity which inactivates a range of biologically active peptides. It is a tumor-specific antigen expressed on cells of lymphoblastic, Burkitt's and follicular germinal center lymphoma and on cells from patients with CML. CD10 has been used as a positive immunohistochemical marker in the diagnosis of human ALL and renal cell carcinomas.\n\nApplications:\nSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000\nImmunohistochemistry (formalin fixed paraffin embedded): 1:20-1:30\nImmunohistochemistry: Frozen\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: \nTonsil\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

C2261-50B

Size

500ul

Applications

IHC, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid, 0.05% sodium azide.

P Type

Mab

Purity

Supernatant

Isotype

IgG1

References

1. Carrel et al. Melanoma Res 3: 319, 1993. 2. Haralambidou et al. J Clin Pathol 40: 490, 1987. 3. Kiyokawa et al. Clin Exp Immunol 79: 322, 1990. 4. Scheuermann et al. Leukemia and Lymphoma 18: 385, 1995. 5. Cutrona et.al British J Cancer 86; 1776-1785 (2002).

Additional Info

Recognizes human CD10.