CD100 (Semaphorin-4D, A8, BB18, GR3, SEMA4D, C9orf164, CD100, SEMAJ)

CD100, a 150kD homodimer, is a novel semaphorin that induces B cells to aggregate and improves their viability in vitro. Semaphorins are neuronal chemorepellants that direct pioneering neurons during nervous system development. CD100 modifies CD40-CD40L B cell signaling by augmenting B cell aggregation and survival and down regulating CD23 expression. CD72 is a lymphocyte receptor for the class IV semaphorin CD100, which is involved in regulating B cell signaling. CD100 is expressed on resting and PHA stimulated T cells. The protein is weakly expressed on NK cells, EBV transformed B cells, monocytes and tumor/peripheral blood T cell lines, and at higher density on activated T cells and B cells.\n\nApplications:\nSuitable for use in Flow Cytometry, Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nFlow Cytometry: (same/less than)2ug/10e5 cells in 100ul\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

C2445-25G

Size

25ug

Applications

FC, IP, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

P Type

Mab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG1,k

References

1. Hall, K., et al. 1996. PNAS. 93:11780. 2. Mizrahi, S., et al. 2007. PLoS One. 2(9):e818. 3. Yoshino, N., et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC) 4. Schlossman, S., L. Bloumsell, W. Gilks, et al., eds. 1995. Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press, New York. 5. Bougeret, C., I.G. Mansur, H. Dastot, et al. 1992. Increased surface expression of a newly identified 150 kDa dimer early after

Additional Info

Recognizes human CD100.