CD104 (CD104 Antigen, Integrin beta-4, Integrin beta4, ITGB4, AA407042, C230078O20) (PE)

Mouse integrin beta 4 (CD104) is a 200kD glycoprotein that can associate with integrin alpha 6 (CD49f) to form a heterodimer on the cell surface. Expression of CD104 is localised to the basal region of a wide variety of epithelial cells. In mice, CD104 is also expressed on peripheral neurones, carcinoma cell lines and a subset of endothelial cells. The alpha 6 beta 4 (CD49f/CD104) integrin has been identified as a component of hemidesmosomes in stratified squamous epithelium where it plays an important role in the adhesion of epithelia to basement membrane. Studies suggest that the CD49f/CD104 integrin is involved in cell matrix adhesion but expression of the CD49f/CD104 integrin in endothelial cells is thought to have a different function.\n\nApplications:\nSuitable for use in Flow Cytometry. Other applications not tested.\n\nRecommended Dilution:\nFlow Cytometry: Neat-1:5; 10ul labels 10e6 cells in 100ul\nOptimal dilutions to be determined by the researcher.\n\nRecommended Negative Control:\nI1904-82H1: IgG2a, Rat Negative Control (PE)\n\nStorage and Stability:\nMay be stored at 4 degrees C before opening. DO NOT FREEZE! Stable at 4 degrees C as an undiluted liquid. Dilute only prior to immediate use. Stable for 12 months at 4 degrees C. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

C2446-02H

Size

100Tests

Applications

FC

Hosts

Rat

Reactivities

Mouse

Form

Supplied as a lyophilized powder from PBS, pH 7.4, 1% BSA, 5% sucrose, 0.09% sodium azide. Labeled with R-Phycoerythrin (PE). Reconstitute with 1ml ddH2O.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG2a

References

1. Kennel, S.J. et al. (1992) The beta 4 subunit of the integrin family is displayed on a restricted subset of endothelium in mice. J. Cell. Sci.101:145-150. 2. Kennel, S.J. et al. (1989) Analysis of the Tumor-associated Antigen TSP-180.Indentity with alpha 6-beta 4 in the integrin superfamily. J. Biol. Chem. 264:15515-15521. 3. Moik, D.V. et al., J Cell SCi. 124:414-21 (2011)

Additional Info

Recognizes mouse CD104.