CD104 (Integrin beta4) (Azide Free)

Human beta4 integrin is also known as CD104. It is a 205kD glycoprotein. which associates with the alpha6/beta4 complex. CD104 is expressed on epithelial cells, Schwann cells and various tumor cell lines.\n\nApplications:\nSuitable for use in Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Western Blot. and Immunofluorescence Not suitable for use with paraffin sections in Immunohistochemistry Other applications not tested.\n\nRecommended Dilutions:\nFlow Cytometry: 1:25-1:100. Use 10ul to label 10e6 cells in 100ul. \nImmunohistochemistry (Frozen): 1:500-1:1000\nOptimal working dilutions to be determined by researcher.\n\nRecommended Positive Control (IHC):\nHuman Tonsil\n\nRecommended Secondary Reagents:\nI1904-65W: IgG, F(ab

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SPECIFICATIONS

Catalog Number

C2446-05A

Size

500ug

Applications

FC, IF, IHC, IP, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in normal saline. No preservatives added.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

1. Kennel, S.J., et al., Second generation monoclonal antibodies to the human integrin alpah6 beta4. Hybridoma 9: 243-254 (1990). 2. Barbaro,V. et al (2009) Reconstruction of a human hemicornea through natural scaffolds compatible with the growth of corneal epithelial stem cells and stromal keratocytes. Mol. Vis. 15: 2084-93. 3. Martel, C.J. & Aasted, B. (2009) Characterization of antibodies against ferret immunoglobulins, cytokines and CD markers. Vet Immunol Immunopathol. 132:109-15. 4. Smith, K. et al. (2011) Mono- and tri-cationic porphyrin-monoclonal antibody conjugates: photodynamic activity and mechanism of action. Immunology. 132: 256-65. 5. Alam, H. et al. (2011) Loss of keratins 8 and 18 leads to alterations in a6b4-integrin-mediated signalling and decreased neoplastic progression in an oral-tumour-derived cell line. J Cell Sci. 124: 2096-106..

Additional Info

Recognizes the human CD104 (beta4 integrin). Recognizes an extracellular epitope on the CD104 molecule. Species Crossreactivity: mustelid and ferret