CD105 (Endoglin, gp160, TGF beta1,3 Receptor) (Biotin)

CD105/endoglin is a disulfide-linked type II transmembrane homodimer that is predominantly expressed on vascular endothelial cells. It is also found on pre-erythroblasts, macrophages and lymphoid and myeloid leukemic cells.2 Both mouse and human CD105 exhibit > 70% sequence homology in their cytoplasmic domains with type III transforming growth factor beta (TGFb) receptor.3 CD105 has been shown to be one of several receptors for the various isoforms of TGFb, which in turn is one of a family of proteins involved in regulation of cell differentiation, migration of cells and control of the immune response.4\n\nApplications:\nSuitable for use in Identification and enumeration of CD105 + cells by Flow Cytometry, Immunocytochemistry and Western Blot. Other applications have not been tested.\n\nRecommended Dilution:\nFlow Cytometry: (same/less than) 1ug/10e6 cells\nImmunocytochemistry: frozen sections\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%) aliquot and store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.\n

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SPECIFICATIONS

Catalog Number

C2446-50D

Size

250ug

Applications

FC, ICC, WB

Hosts

Rat

Reactivities

Mouse

Conjugates

Biotin

Form

Supplied as a liquid in PBS. Conjugated to Biotin.

P Type

Mab

Purity

Purified

Isotype

IgG2a,k

References

1. Ge, A.Z.,, and E.C. Butcher. 1994. Gene 138: 201. 2. Letarte, M., A. Greaves, and S. Vera. 1995. CD105 (endoglin) cluster report. In: Leucocyte Typing V, White Cell Differentiation Antigens. Eds. Schlossman, S., L. Boumsell, W. Gilks, J.M. Harlan, T Kishimoto, C. Morimoto, J. Ritz, S. Shaw, R. Silverstein, T. Springer, T.F. Tedder, and R.F. Todd. Oxford University Press, p. 1756. 3. Berg, e., et al. 1989. Immunol. Rev. 108: 5.\n4. Lastres, P., et al. 1996. J. Cell. Biol. 133: 1109.

Additional Info

Recognizes mouse endoglin, Mr 90kD.