CD105 (Endoglin, gp160, TGF beta1,3 Receptor)

Endoglin is a 190kD homodimeric transmembrane glycoprotein composed of disulfide-linked subunits. The external domain binds TGF-beta1 and -beta3 isoforms with high affinity. Two different isoforms (L and S) of CD105 with capacity to bind TGF-beta have been characterized which differ in the amino acid composition of their cytoplasmic tails. Mutations in the gene encoding endoglin have been linked to the human disease hereditary hemorrhagic telangiectasia type 1 (HHT1) a vascular disorder characterized by localized vascular dysplasia and a tendency towards arteriovenous malformations. Mice expressing a single CD105 allele develop external signs of disease similar to human HHT1, supporting the haploinsufficiency model for HHT1. Mice lacking endoglin die from defective angiogenesis characterized by failure of vascular smooth muscle investment of embryonic blood vessels, suggesting a defect in vascular smooth muscle cell development. Microvessel density (MVD) has been reported to be an independent prognostic indicator of outcome in a variety of human malignancies, with increased MVD correlating with shorter overall and relapse-free survival rates. The MVD counts using anti-CD105 antibody significantly correlated with overall and disease-free survival.\n\nApplications:\nSuitable for use in ELISA, Immunoprecipitation, Western Blot, Flow Cytometry and Immunohistochemistry. Not Suitable for paraffin sections or for inhibition of biological activity. Other applications not tested.\n\nRecommended Dilution:\nELISA: coating and detection\nImmunohistochemistry (Frozen): 1:50.\nFlow Cytometry: 1:50\nWestern Blot: 1:50\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

C2446-52

Size

100ug

Applications

ELISA, FC, IHC, IP, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

1. Wang, J et al; J Natl Cancer Inst 86: 386 (1994). 2. Pichuantes, S et al; Tissue Antigens 50: 265(1997). 3. Kumar, S et al; Cancer Research , 59: 856(1999). 4. Li, C et al; . Int J Cancer , 89: 122(2000). 5. Li, C et al; B J Cancer , 88: 1424(2003). 6. Costello, B et al; Int J Cancer 109: 436(2004).

Additional Info

Recognizes human CD105 (Endoglin).