CD106 (Vascular Cell Adhesion Molecule-1, VCAM-1, INCAM-110)

VCAM-1, the vascular cell adhesion molecule, is a member of the immunoglobulin superfamily, C2 subset. The antigen is induced on human endothelial cells by TNF-alpha, IL-1, IFN-gamma or endotoxin. The ligands for VCAM-1 are integrin alpha4, beta1 (VLA-4) and integrin alpha4, beta7. These integrins are constitutively expressed by thymocytes, lymphocytes and monocytes.\n\nApplications:\nUseful for staining of VCAM-1 expressing endothelial cells. It permits staining of in vitro cultured cells and frozen tissue sections. Furthermore the antibody can be used for Flow Cytometry, immunoassays and Immunoprecipitation.\n\nRecommended Dilution:\nFlow Cytometry: 1:10\nImmunohistology: Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. In vitro cultured cells can be fixed with 1% paraformaldehyde, tissue sections are advised to be fixed for 10 min in pure acetone and followed by incubation for 10 min in chloroform. Incubation with a pretested dilution of the antibody is advised to be followed by a biotin conjugated anti-murine Ig and a further incubation with an enzyme (alkaline phosphatase) conjugated streptavidin. For selection of the most useful dilution in a given situation a test staining with cells or tissue known to express the antigen should be performed. To this end either cultured endothelial cells or a small fresh skin biopsy can be incubated for 4 hours with TNF-alpha (1ng/ml), IL-1 (100U/ml) or LPS (1ug/ml) in tissue culture medium at 37 degrees C. As negative control it is advised to use a control murine IgG1 antibody.\n\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20 degrees C or colder. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C2446-77A

Size

100ug

Applications

FC, IHC, IP

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, containing 0.02% sodium azide and 0.1% BSA.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

1. Polte, T et al; Full length vascular cell adhesion molecule 1 (VCAM-1). Nuclear Acids Res 1990, 18: 5901. 2. Thornhill, M et al; Tumor necrosis factor combines with IL-4 or IFN-gamma to selectively enhance endothelial cells adhesiveness for T cells. The contribution of vascular cell adhesion molecule-1-dependent and -independent binding mechanisms. J Immunology 1991, 146: 592. 3. Kyan-Aung, U et al; VCAM-1 and eosinophyl adhesion to cultured human umbilical vein endothelial cell. Am Review 1991, 5: 445.

Additional Info

Reacts with human VCAM-1