CD106 (Vascular Cell Adhesion Molecule-1, VCAM-1, INCAM-110) (FITC)

VCAM-1 is present on the +/+ 2.4 stromal cell clone, large cells in bone marrow and a subset of thymic macrophages. The stromal cell antigen is also detectable on the EOMA and BMS2 endothelial cell lines. \n\nApplications:\nSuitable for use in Flow Cytometry. Other applications not tested.\n\nRecommended Dilution:\nFlow Cytometry: 1:10, use 10ul of the suggested working dilution to label 10e6 cells in 100ul.\nOptimal working dilutions to be determined by researcher.\n\nRecommended Negative Control: \nI1904-78R3: IgG1 Murine Negative Control (FITC)\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C2446-74G

Size

100Tests

Applications

FC

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

1. Thornhill, M.H. et al. (1991). J. Immunol. 146: 592-598.2. Rosenman, S. J. et al. (1995). Cytokine-induced expression of vascular cell adhesion molecule-1 (VCAM-1) by astrocytes and astrocytoma cell lines. J. Immunol. 154

Additional Info

Recognizes human VCAM-1, a 110kD molecule whose ligand is VLA4. The antigen is expressed on activated endothelial cells and on some tissue macrophages, bone marrow fibroblasts and myoblasts.