CD106 (Vascular Cell Adhesion Molecule-1, VCAM-1, INCAM-110) (PE)

Human CD106 is an endothelial adhesion molecule that binds to a4 b1 & a4b7 integrins (VLA-4) and promotes adhesion of lymphocytes, monocytes, eosinophils and basophils. CD106 is also found on follicular dendritic cells, interdigitating recticulum cells and Kupffer cells.\n\nApplications: \nSuitable for use in Flow Cytometry and blocks lymphocyte adhesion to CD106.. Other applications not tested.\n\nRecommended Dilution:\nFlow Cytometry: 1:50\nOptimal dilutions to be determined by the researcher.\n\nFlow Cytometry Protocol:\n1. Cultured human umbilical cord vein endothelialcells were stimulated 4 hours with 10ng/ml Phorbol 12-Myristate 13-Acetate. \n2. Five x 10e5 cells per tube were harvested, washed and incubated 45 minutes on ice with 80ul of C2446-71J at a 1:50 dilution. \n3. Cells were washed three times, fixed and analyzed on a BD FACstar plus. \n4. Cells stained positive with a mean shift of 1.36 log10 fluorescent units when compared to a Mouse IgG1/R-PE negative control at a similar concentration. Binding was 93% blocked when pre incubated 10 minutes with 20ul of 0.5 mg/ml of C2446-71F.\n\nStorage and Stability:\nMay be stored at 4 degrees C before opening. DO NOT FREEZE! Stable at 4 degrees C as an undiluted liquid. Dilute only pror to immediate use. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity.

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SPECIFICATIONS

Catalog Number

C2446-71J

Size

120Tests

Applications

FC

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in 50mM Sodium phosphate pH 7.5, 500mM potassium chloride, 150mM NaCl,, 0.2% BSA, 0.04% sodium azide, 15% glycerol.

P Type

Mab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG1

References

1. M.H. Thornhill, D.O. Haskard, (1991) J Immunol 146: 592-598. 2. Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 1764-1767. 3. M. Nagata, et al, (1995) J Immunol 155: 2194-2202. 4. H.E. Chuluyan, et al, (1995) J Immunol 155: 3135-314.

Additional Info

Recognizes the VCAM-1 molecule (CD106) which is involved with leukocyte adhesion.