CD106 (Vascular Cell Adhesion Molecule-1, VCAM-1, INCAM-110) (PE)

VCAM-1 is an adhesion molecule and a major mediator of the inflammatory response. It is expressed on activated microvascular endothelial cells in response to signals arising from immune responses in infection, graft rejection, tumor recognition and killing. The complementary binding ligand for VCAM-1 is VLA-4/CD49d. In addition to VCAM-1, VLA-4 also recognizes the extracellular matrix molecule fibronectin. This pairing of VCAM-1 and VLA-4 is able to provide a second signal (i.e., non-antigen specific) for T cell stimulation, such as that seen in transplantation. The monoclonal antibody has been used in transplant studies to suppress cardiac rejection and induce long-term cardiac graft survival. In addition to inflammatory responses, VCAM-1 has a significant role in hemopoiesis through its ability to retain lymphocyte and myeloid precursors on stromal cells in the marrow and lymphoid organs. CD106/VCAM-1 exists as an integral membrane protein. The monoclonal antibody immunoprecipitates a peptide that gives a single band on SDS-PAGE gels with an apparent Mr of ~100kD under reducing conditions and 92kD under nonreducing conditions (1-5).\n\nApplications: \nSuitable for use in Immunofluorescence, Flow Cytometry, Immunoprecipitation, Immunohistochemistry.\nOther applications not tested.\n\nRecommended Dilution:\nFlow Cytometry: <0.2ug/10e6 cells\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nStorage and Stability:\nMay be stored at 4 degrees C before opening. DO NOT FREEZE! Stable at 4 degrees C as an undiluted liquid. Dilute only prior to immediate use. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity.\n\nNote: We do not recommended adding glycerol as a cryopreservative.

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SPECIFICATIONS

Catalog Number

C2446-79D

Size

100ug

Applications

FC, IF, IHC, IP

Hosts

Rat

Reactivities

Mouse

Form

Supplied as a liquid in PBS, 0.1% sodium azide and a stabilizing agent. Labeled with PE.

P Type

Mab

Purity

Purified

Isotype

IgG1,k

References

1. Miyake, K., I.L. Weissman, J.S. Greenberger and P. W. Kincade; J. Exp. Med. 173:599 (1991). 2. Miyake, K., et al.; J. Cell. Biol. 114(3):557 (1991).\n3. Schlegel, P.G., et al.; J. Immunol. 155:3856 (1995). 4. Bergese, S., et al.; Am J. Phathol. 146:989 (1995). 5. Orosz, C.G., et al.; Transplantation 56:453 (1993).

Additional Info

Recognizes mouse CD106/VCAM-1 (~Mr 100kD).