CD106 (Vascular Cell Adhesion Protein 1, V-CAM 1, VCAM-1, Vcam1)

VCAM-1 (~81kD) is a member of a subclass of the immunoglobulin superfamily (IgSF). IgSF members are ligands for integrins. Cell adhesion molecules (CAMs) have important roles in the immune response, immune surveillance and cell-cell recognition, especially in leukocyte-endothelial cell adhesion. CAMs on the surface of leukocytes and endothelial cells are actively involved in the recruitment of specific leukocyte subsets into different tissues. VCAM-1 is expressed on inflamed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflamed tissue. Cell adhesion molecules, like VCAM-1, are upregulated on cerebral vessels during inflammatory conditions of the central nervous system such as experimental autoimmune encephalomyelitis (EAE), a model system for multiple sclerosis. Administration of this monoclonal antibody has been shown to inhibit or diminish clinical or pathological signs of EAE. VCAM-1 is a receptor for encephalomyocarditis virus on murine vascular endothelial cells. Expression of VCAM-1 on vascular endothelial cells is induced by TNF-alpha, IL-1, IFN-gamma or endotoxin. VCAM-1 is a ligand for the integrins alpha4beta1 (VLA-4) and alpha4beta7 (LPAM-1). These integrins are constitutively expressed by thymocytes, lymphocytes and monocytes. VCAM-1/VLA-4 interaction may play a pathophysiological role in immune responses and as well as in leukocyte emigration to sites of inflammation.\n\nApplications:\nSuitable for use in Flow Cytometry, Immunohistochemistry, Functional Assays, Immunofluorescence and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nFlow Cytometry: 1:50\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

C2447-02Q

Size

100ug

Applications

FC, IF, IHC, IP

Hosts

Rat

Reactivities

Mouse

Form

Supplied as a liquid in PBS, 0.1% BSA.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

1. Engelhardt B et al; The development of experimental autoimmune encephalomyelitis in the mouse requires alpha4-Integrin but not alpha4beta7-Integrin. J Clin Invest 1998, 102: 2096. 2. Vajkoczy P et al; Alpha4-integrin-VCAM-1 binding mediates G protein-independent capture of encephalitogenic T cell blasts to CNS white matter microvessels. J Clin Invest 2001, 108: 557. 3. Kuligowski M et al; Antimyeloperoxidase antibodies rapidly induce {alpha}4-integrin-dependent glomerular neutrophil adhesion. Blood 2009, 113: 6485. 4. Kneilling M et al; Direct crosstalk between mast cell TNF and TNFR1-expressing endothelia mediates local tissue inflammation. Blood 2009, 114: 1696.

Additional Info

Recognizes mouse VCAM-1.