CD11 (b/c) (Integrin alpha-M/alpha-X Cell Surface Glycoprotein, MAC-1 alpha Subunit, Cell Surface Glycoprotein p150, 95 alpha Chain, CR-3 alpha Chain, Leukocyte Adhesion Receptor, MO1 Leukocyte Adhesion Receptor p150, 95, CD11b/c Antigen, CD11 b/c)

CD11b is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement coated particles. It is identical to CR3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the RGD peptide in C3b. CD11b is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. The Mac1 CD11b antigen is present on macrophages, granulocytes, natural killer cells, blood monocytes. CD11b is expressed on 8% spleen cells, 44% bone marrow cells and less than 1% of thymocytes and is commonly used as a microglial marker in nervous tissue.\n\nApplications: \nSuitable for use in Western Blot, Flow Cytometry, Immunofluorescence, Immunocytochemistry and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: 2-4ug/ml. A doublet is seen at ~95kD and 103kD. A weaker doublet is also seen at ~130kD and 160kD.\nFlow Cytometry: 1:50-1:200\nImmunofluorescence (IC): 1:50-1:200\nImmunohistochemistry (Formalin fixed paraffin): 5-10ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: \nRAW 264.7 cell lysate\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

C2262-02H

Size

100ul

Applications

FC, IF, IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse

Form

Supplied as a liquid in Tris-glycine, 150mM sodium chloride, pH 7.2, 0.05% sodium azide.

P Type

Pab

Purity

Purified by peptide affinity chromatography.

References

1. Chakrabarty P, Jansen-West K, Beccard A, et al. Massive gliosis induced by interleukin-6 suppresses A{beta} deposition in vivo: evidence against inflammation as a driving force for amyloid deposition. FASEB J 2009:fj.09-141754. [PMID: 19825975]. 2. Chakrabarty P, Ceballos-Diaz C, Beccard A et al. IFN-gamma promotes complement expression and attenuates amyloid plaque deposition in amyloid beta precursor protein transgenic mice. J Immunol. 2010 May 1;184(9):5333-43 [PMID: 20368278].

Additional Info

Recognizes mouse CD11 (b/c). Species Crossreactivity: human

Alternative Names

Integrin alpha-M/alpha-X Cell surface glycoprotein, MAC-1 alpha subunit, Cell surface glycoprotein p150, 95 alpha chain, CR-3 alpha chain, Leukocyte adhesion receptor, MO1 Leukocyte adhesion receptor p150, 95, CD11b/c antigen, cd11 b/c