CD115 (c-fms, Fms, CSF-1R, M-CSFR) (BSA & Azide Free)

CD115 is also known as c-fms, macrophage colony stimulating factor receptor, or CSF-1R. It exists in immature (gp130 c-fms) and mature (gp150 c-fms) forms. CSF-1R exhibits an intrinsic tyrosine-specific protein kinase activity. It is expressed on the cells of the mononuclear phagocyte lineage. CSF-1R is an integral transmembrane glycoprotein consisting of a 512 amino acid extracellular domain that includes the CSF-1 binding site, a single 25 amino acid membrane-spanning segment, and a 435 amino acid cytoplasmic portion that includes the tyrosine kinase domain.\n\nApplications: \nSuitable for use in blocking ligand binding and ligand dependent proliferation, inhibition of CSF-1 independent cell growth, Immunofluorescence, and Immunohistochemistry. Other applications have not been tested.\n\nRecommended Dilution:\nImmunohistochemistry: Acteone-fixed frozen.\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: Tonsil\n\nSource: Ascites\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C2447-50X

Size

100ug

Applications

IF, IHC

Hosts

Rat

Reactivities

Hum

Form

Supplied as liquid in PBS, pH 7.4. Also available with BSA and azide. See cat # C2447-50.

P Type

Mab

Purity

Purified by ammonium sulfate precipitation.

Isotype

IgG1,k

References

1. Ashmun, R.A., et al., Blood 73(3): 827-37 (1989). 2. Sherr, C.J., et al., Blood 73(7): 1786-93 (1989). 3. Schlossman, S.F., et al., Leucocyte Typing V p988-989, Oxford Univ. Press (1993).

Additional Info

Recognizes proteins of 130kD & 150kD, identified as immature (gp130c-fms) and mature (gp150c-fms) forms of human CD115. Blocks ligand binding and ligand-dependent proliferation. Inhibits CSF-1-independent growth of cells expressing oncogenic, mutant CSF-1R. Does not induce internalization or turnover of CSF-IR. Species crossreactivity: Does not react with mouse and feline.