CD115, phosphorylated (Tyr809) (c-fms, Fms, CSF-1R, M-CSFR)

Macrophage-colony stimulating factor (M-CSF, CSF-1) receptor is an integral membrane tyrosine kinase encoded by the c-fms proto-oncogene. MCSF receptor is expressed in monocytes (macrophages and their progenitors) and drives growth and development of this blood cell lineage (1-3). Binding of M-CSF to its receptor induces receptor dimerization, activation and autophosphorylation of cytoplasmic tyrosine residues used as docking sites for SH2-containing signaling proteins (4). There are at least five major tyrosine autophosphorylation sites. Tyrosine 723 (Tyr721 in mouse) is located in the kinase insert (KI) region. Phosphorylated tyrosine 723 binds the p85 subunit of PI3 kinase as well as PLC-gamma2 (5). Phosphorylation of Tyr809 in the receptor provides a docking site for Shc adaptor protein (5). Overactivation of this receptor can lead to a malignant phenotype in various cell systems (6). The activated M-CSF receptor has been shown to be a predictor of poor outcome in advanced epithelial ovarian carcinoma (7) and breast cancer (8).\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: 1:1000\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

C2447-49

Size

100ul

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse

Form

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

P Type

Pab

Purity

Purified by Protein A and peptide affinity chromatography.

Isotype

IgG

References

1. Stanley, E.R., et al., Nature 274: 168-170 (1978). 2. Byrne, P.V., et al., J. Cell. Biol. 91: 848-853 (1981). 3. Bourette, R.P., et al., Growth Factors 17: 155-166 (2000). 4. Novak, U., et al., Oncogene 13: 2607-2613 (1996). 5. Bourette, R.P., et al., EMBO J. 16: 5880-5893 (1997). 6. Morley, G.M., et al., Oncogene 18: 3076-3084 (1999). 7. Toy, E.P., et al., Gynecol. Oncol. 80: 194-200 (2001). 8. Maher, M.G., et al., Clin. Cancer Res. 4: 1851-1856 (1998).

Additional Info

Recognizes endogenous levels of human M-CSF receptors at ~175kD only when phosphorylated at Tyr809. Slight crossreactivity with activated KDR and PDGF receptor. Does not crossreact with other unrelated tyrosine-phosphorylated proteins. Species Crossreactivity: mouse.