CD117 (c-kit, Stem Cell Factor, SCF Receptor, Steel Factor Receptor, Mast Cell Growth Factor, MGF) (BSA & Azide Free)

CD117 or c-kit, a transmembrane receptor tyrosine kinase, is a receptor for a growth factor encoded by the steel locus and known as steel factor (SLF), stem cell factor (SCF), mast cell growth factor MGF or KIT Ligand (KL). The binding of the stem cell factor (SCF) to the c-kit-encoded receptor tyrosine kinase (Type III) stimulates a variety of biochemical responses that culminate in cellular proliferation, migration or survival. c-kit plays an important role in hematopoiesis, melanogenesis and gametogenesis. The antigen is expressed in many tissues and cells: 0.6-3.4% of normal bone marrow cells which comprise BFU-E, CFU-GEMM, CFU-GM and CFU-G. 19-51% of CD34+ bone marrow cells co-express c-kit. c-kit/SCF is involved in development of several lineages of stem cells, such as germ cells, neural crest-derived melanocytes, and hematopoietic precursor cells.\n\nApplications: \nSuitable for use in Immunofluorescence and Immunohistochemistry. Other applications have not been tested.\n\nRecommended Dilution:\nImmunohistochemistry: Frozen or fixed in methanol/Carnoy

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SPECIFICATIONS

Catalog Number

C2448-07X

Size

100ug

Applications

IF, IHC

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.4, before the addition of glycerol to 40%. No preservatives and no stabilizing proteins added. Also available with BSA and azide. Use cat# C2448-07.

P Type

Mab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG2a

References

1. Blechman, J.M., et al., J. Biol. Chem. 268(6): 4,399-4,406 (1993). 2. Blechman, J.M., et al., Cell 80(1): 103-113 (1995).

Additional Info

Recognizes human CD117/c-kit at 145kD. Does not interfere with the binding of SCF to c-kit. Precipitates both the unoccupied as well as the occupied form of c-kit.