CD117 (c-kit, Stem Cell Factor, SCF Receptor, Steel Factor Receptor, Mast Cell Growth Factor, MGF)

The c-kit proto-oncogene is the cellular homologue of the feline sarcoma v-kit oncogene. It encodes a type III transmembrane receptor tyrosine kinase also known as CD117 or stem cell factor receptor (SCFR). c-Kit signaling is activated when a ligand known as steel factor or stem cell factor (SCF) binds to the receptor. Interactions between specific phosphotyrosine residues of activated receptor tyrosine kinases (RTKs) and Src homology 2 (SH2) domains define cellular\ngrowth and differentiation signals. It has been reported that SCF stimulates the association of the p85 subunit of PI3K with the phosphorylated tyrosine-721 residue within wild type kit. Subsequently, PI3K contributes to the activation of Akt and JNKs. c-Kit is necessary for the development of hematopoietic progenitors, mast cells, germ cells, melanocytes, and the interstitial cells of Cajal (ICC). Oncogenic c-kit mutations have been implicated in neoplasms arising from these stem cell lineages, such as gastrointestinal stromal tumor (GIST). The mature, phosphorylated form of c-Kit is a glycosylated protein with a molecular weight of ~145kD, while the immature form is ~125kD.\n\nApplications:\nSuitable for use in Western Blot and Immunohistochemistry. Other applications have not been tested.\n\nRecommended Dilution:\nWestern Blot: 1-3ug/ml\nImmunohistochemistry: 5-10ug/ml\nNote: Prior to immunohistochemistry with paraffin-embedded tissue sections, heat induced epitope retrieval with EDTA, pH 8.0.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C2448-04J

Size

100ug

Applications

IP, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide.

P Type

Pab

Purity

Purified by epitope affinity chromatography.

Additional Info

Reacts with the human c-Kit (CD117 or stem cell factor receptor (SCFR)) oncoprotein. On Western blots, this antibody identifies a ~145kD band representing the mature (phosphorylated, glycosylated) form of c-Kit, a ~125kD band for the immature form, and a ~95kD band representing the soluble form. Reactivity has been confirmed with human GIST-882 and EWS-502 cell lysates.