CD117 (CD117 Antigen, c Kit, c-Kit, KIT, Mast/stem Cell Growth Factor Receptor, PBT, Proto-oncogene Tyrosine Protein Kinase Kit, Stem Cell Factor Receptor, SCF Receptor, SCFR, v-Kit Hardy Zuckerman 4 Feline Sarcoma Viral Oncogene Homolog)

The CD117/c-kit antigen is the cell-surface receptor for Stem Cell Factor (SCF). Together these molecules constitute a ligand/receptor pair which functions to maintain normal hematopoiesis in the adult.1-3 Signaling through SCF/c-kit has an important role in stimulating myeloid and erythroid production of primitive hematopoietic progenitor cells.2 A variety of cytokines act synergistically with SCF to stimulate proliferation and differentiation of bone marrow progenitor cells. For example, SCF plus IL-7 can stimulate the combined myeloid and B-cell differentiation of uncommitted hematopoietic progenitor cells.2 SCF/c-kit also participates in erythropoiesis in both the bone marrow and spleen.1 Loss-of-function mutations for SCF/c-kit lead to a variety of pleiotropic developmental defects, while gain-of-function mutations can lead to constitutive activation of the kit receptor and links to cancer.3 Examples of the former are mast cell deficiency and severe macrocytic anemia, while the latter mutation has been identified in mastocytomas.3 Anti-CD117 mAbs have been used to block the function of the c-kit receptor in a variety of studies. These include the blockade of hematopoiesis1, 4 and disruption of melanocyte development.5\n\nApplications:\nSuitable for use in Flow Cytometry. Other applications not tested.\n\nRecommended Dilution:\nFlow Cytometry: 1:50-1:200; 10ul labels 10e6 cells in 100ul\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C2448-05E1

Size

250ug

Applications

FC

Hosts

Rat

Reactivities

Mouse

Form

Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG2b

References

1. Broudy, V.C., et al, 1996. Blood 88:75.\n2. Veiby, O.P., S. D. Lyman and S. E. Jacobsen. 1996. Blood 87:3117.\n3. Sudo, T., et al. 1995. Oncogene 11:2469.\n4. Yoshida, H., et al. 1996. Development 122: 1207.

Additional Info

Recognizes mouse CD117.