CD117 (CD117 Antigen, c-Kit, KIT, Mast/stem Cell Growth Factor Receptor, PBT, Proto-oncogene c-Kit, SCFR, Tyrosine-protein Kinase Kit)

CD117, a 145kD Type III transmembrane Tyrosine Kinase receptor, is a novel member of Ig superfamily. It is a Human homolog of proto-oncogene c-Kit and is expressed in tissue mast cells and hematopoietic stem and progenitor cells. It consists of an extracellular domain with Ig-like repeats and a Tyrosine Kinase domain, a putative transmembrane region and a cytoplasmic domain. CD117 functions as receptor for MGF/SCF resulting in maturation of hemopoietic and primordial germ cell precursors and melanocytes during embryonic development. CD117 gets autophosphorylated and activates signaling pathways including PI3K/Akt, MAPK and JNK affecting multiple cellular functions including cell survival, proliferation, adhesion and differentiation. Mutations in KIT are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous leukemia and piebaldism.\n\nApplications:\nSuitable for use in Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nImmunohistochemistry (paraffin): 1:50-1:100\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nTonsil\n\nCellular Localization: \nCytoplasmic and cell membrane\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

C2448-03Q

Size

500ul

Applications

IHC

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in 0.05% sodium azide.

P Type

Pab

Purity

Serum

Isotype

IgG

References

1. Arber et al. Hum Pathol 29: 498, 1998. 2. Rajpert et al. J Pathol 178: 166, 1996. 3. Arber et al. Hum Pathol 29: 498, 1998. 4. Rajpert et al. J Pathol 178: 166, 1996. 5. Zeng et.al Blood 105 (1) 226-232 (January 1, 2005). 6. Catalano et.al JBC 279 (45) 46706-46714 (November 5, 2004). 7. Yarden et.al EMBO J 6 (11) 3341-3351 (1987). 8. Kitamura et.al Cell Mol Life Sci 61: 2924-2931 (2004).

Additional Info

Recognizes human CD117.

Alternative Names

v-kit Hardy-Zuckerman 4 Feline Sarcoma Viral Oncogene Homolog, EC=2.7.10.1