CD11a (Integrin Alpha L, ITGAL, Leukocyte Adhesion Glycoprotein LFA1 Alpha Chain, LFA1A, Ly15, Ly21, Lymphocyte Function Associated Antigen Type 1 alpha, p180)

Integrin subunit aL/CD11a is a 180kD type I TM glycoprotein that interacts only with Integrin b2/CD18 to form LFA-1, a leukocyte adhesion protein which binds endothelial cell ICAM. Human Integrin aL contains a 1064aa extracellular domain (ECD), a 20aa TM sequence and a 58aa cytoplasmic domain. The ECD contains seven repeats that form a beta-propeller structure and one inserted vWA domain (I domain) containing a metal ion-dependent adhesion site (MIDAS). Human and mouse Integrin aL ECD share 74aa identity. A second isoform has a 53aa insert in the ECD.\n\nApplications:\nSuitable for use in Flow Cytometry. Other applications not tested.\n\nRecommended Dilution:\nFlow Cytometry: 2.5ug labels 10e6 cells\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C2262-52F3

Size

100ug

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.5mg/ml.

P Type

Mab

Purity

Purified by Protein A/G affinity chromatography.

Isotype

IgG2A

Additional Info

Recognizes human CD11a. No cross-reactivity with recombinant human Integrin a1, a2, a3, a4, a5, a6, a8, a9, a10, a11, aM, aV, aX, Integrin b3, Integrin a6(x1)+b4, a7(x2)+b1, aD+b2, aE+b7, recombinant mouse Integrin a2b or aL.