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CD11a (Leukocyte Function Associated Antigen, LFA-1, Integrin aL) (Azide Free)

Cat no: C2262-03M

CD11a (Leukocyte Function Associated Antigen, LFA-1, Integrin aL) (Azide Free)

Recommended Secondary Reagents: Goat anti-mouse IgG: HRP conjugate (rat absorbed), IgG Goat anti mouse IgG: RPE (rat adsorbed)\n\nFusion Partners: Spleen cells from immunized BALB/c mice were fused with cells of the PAI mouse myeloma cell line.\n\nRecommended Negative Controls: Mouse IgG2a Negative Control: Azide Free\n\nApplications:\nFlow Cytometry: 1:50-1:100\nImmunohistochemistry (frozen)\nImmunoprecipitation\nBlocking\nOptimal working dilutions to be determined by researcher.\n\nFlow Cytometry: Use 10ul of the suggested working dilution to label 10e6 cells in 100ul.

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SPECIFICATIONS

Catalog Number

C2262-03M

Size

1mg

Applications

FC, IHC, IP

Hosts

Mouse

Reactivities

Rat

Form

Supplied as a liquid in PBS.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG2a

References

1. Tamatani, T., Kotani, M. and Miyasaka, M. (1991). Eur. J. Immunol. 21: 627-633.2. Tamatani, T., Kotani, M., Tanaka, M. and Miyasaka, M. (1991). Eur. J. Immunol. 21: 855-858.

Additional Info

Reacts with the rat LFA-1 alpha chain (160-170kD) which is associated with the beta chain (CD18; 95-100kD). LFA-1 is one of the leucocyte integrins (beta 2 integrin) which plays an important role in leucocyte physiology. Functionally, this monoclonal antibody inhibits homotypic aggregation of PHA blasts.

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