CD11a (Leukocyte Function Associated Antigen, LFA-1, Integrin aL)

Human CD11a (alpha L integrin) complexes with CD18 (beta2 integrin) to form the LFA-1 heterodimer adhesion molecule which binds to three ICAM's 1, 2, and 3, also designated CD54, CD102, and CD50 respectively. LFA-1 is expressed on lymphocytes, monocytes and neutrophils and plays a role in cell-cell adhesion.\n\nApplications:\nSuitable for use in Flow Cytometry, Western Blot and Antibody Blocking. Other applications not tested.\n\nRecommended Dilution:\nAntibody blocking: 5-10ug/ml blocks binding of ICAM-1 and ICAM-3 to LFA-1.\nFlow cytometry: 5ug/ml\nOptimal dilutions to be determined by the researcher.\n\nFlow cytometry Protocol:\n1. Five x 10e5 cultured HPB-MLT human tumor cells were washed and incubated 45 minutes on ice with 80ul of C2262-02C at 5ug/ml. \n2. Cells were washed twice and incubated with secondary reagent Goat anti-Mouse IgG/FITC. \n3. Cells were then washed three times, fixed and analyzed by FACS.

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SPECIFICATIONS

Catalog Number

C2262-02C

Size

100ug

Applications

FC, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.2, 0.5mg/ml gentamicin sulfate.

P Type

Mab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG2a

References

1. I. Dransfield & N. Hogg, (1989) EMBO J 12: 3759-3765. 2. Leukocyte Typing V (S.F. Schlossman,et al, eds.) Oxford University Press, Oxford, (1995) p. 1581-1592. 3. R.C. Landis, et al, (1994) J Cell Biol 126: 529-537. C.L. Holness, et al, (1995) J Biol Chem 270: 877-884.

Additional Info

Recognizes the I domain of the human integrin alpha L subunit (CD11a) of ~180kD.