CD11b (Cell Surface Glycoprotein MAC1 Alpha Subunit, Complement Component Receptor 3 Alpha, CR3A, ITGAM, MAC1A, MO1A, Neutrophil Adherence Receptor)

CD11b cell surface antigen (also known as the alpha M integrin chain or MAC-1) is a differentiation antigen expressed by granulocytes, monocytes, NK cells and tissue macrophages. The expression of CD11b increases during monocyte maturation and expression levels vary on tissue macrophages. Peritoneal macrophages are reported to express higher levels of CD11b than splenic macrophages. \n\nApplications:\nSuitable for use in Flow Cytometry, Immunofluorescence Functional Assays, Immunohistochemistry and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilutions:\nFlow Cytometry: 1:50-1:100\nImmunohistochemistry: Frozen and PLP fixed paraffin embedded tissue. \nOptimal dilutions to be determined by the researcher. \n\nHybridoma: \nNS1/1.Ag4.1 myeloma cells with spleen cells from DA rats\n\nRecommended Negative Control:\nI1904-82V4: IgG2b, Rat Negative Control\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

C2262-37A

Size

500ug

Applications

FC, IF, IHC, IP

Hosts

Rat

Reactivities

Hum, Mouse, Rabbit

Form

Supplied as a liquid in PBS. No preservative added.

P Type

Mab

Purity

Purified by Protein G affinity chromatography. Endotoxin: (same/less than)0.01EU/ug

Isotype

IgG2b

References

1. Springer, T. et al. (1978) Monoclonal xenogeneic antibodies to murine cell surface antigens: identification of novel leukocyte differentiation antigens. Eur. J. Immunol. 8:539-551, 2. Springer, T. et al. (1979) Mac-1: a macrophage differentiation antigen identified by a monoclonal antibody. Eur. J. Immunol. 9:301-306, 3. Beller, D.I. et al. (1982) Anti-Mac-1 selectively inhibits the mouse and human type three complement receptor J. Exp. Med. 156:1000-1009, 4. Whiteland, J.L. et al. (1995) Immunohistochemical detection of T cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies. J. Histochem. Cytochem. 43:313-320, 5. Klingel, K. et al. (2003) Beta2-microglobulin-associated regulation of interferon-gamma and virus-specific immunoglobulin G confer resistance against the development of chronic coxsackievirus myocarditis. Am. J. Pathol. 162(5):1709-1720, 6. Welt, F.G. et al. (2000) Neutrophil, not macrophage, infiltration precedes neointimal thickening in balloon-injured arteries. Arterioscler Thromb Vasc Biol. 20: 2553-2558.

Additional Info

Recognizes mouse CD11b cell surface antigen. Reported to block iC3b binding to its

Alternative Names

Cell Surface Glycoprotein MAC1 Alpha Subunit, Complement Component Receptor 3 Alpha, CR3A, ITGAM, MAC1A, MO1A, Neutrophil Adherence Receptor