CD11b (Cell Surface Glycoprotein MAC1 Alpha Subunit, Complement Component Receptor 3 Alpha, CR3A, ITGAM, MAC1A, MO1A, Neutrophil Adherence Receptor)

Applications: \nSuitable for use in Flow Cytometry and Immunohistochemistry (paraffin-embedded sections). Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C2262-39F

Size

100ug

Applications

FC, IHC

Hosts

Rat

Reactivities

Hum, Mouse, Rabbit

Form

50% Glycerol, PBS

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG2b

References

This product has been used in:Whiteland JL et al. Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies. J Histochem Cytochem 43:313-20 (1995). <PUBMED:7868861>Smet EG et al. Mab. 198: a monoclonal antibody recognizing the complement type 3 receptor (CR3) in the rabbit. Immunology 59:419-25 (1986). <PUBMED:3793137>Lopez AF et al. Epitope diversity of monoclonal antibodies revealed by cross-species reactivity. Mol Immunol 21:371-4 (1984). <PUBMED:6204196>Ho MK & Springer TA Mac-1 antigen: quantitative expression in macrophage populations and tissues, and immunofluorescent localization in spleen. J Immunol 128:2281-6 (1982). <PUBMED:7037963>Kurzinger K & Springer TA Purification and structural characterization of LFA-1, a lymphocyte function-associated antigen, and Mac-1, a related macrophage differentiation antigen associated with the type three complement receptor. J Biol Chem 257:12412-8 (1982). <PUBMED:6749860>Ault KA & Springer TA Cross-reaction of a rat-anti-mouse phagocyte-specific monoclonal antibody (anti-Mac-1) with human monocytes and natural killer cells. J Immunol 126:359-64 (1981). <PUBMED:7451976>Springer T et al. Mac-1: a macrophage differentiation antigen identified by monoclonal antibody. Eur J Immunol 9:301-6 (1979). <PUBMED:89034>Springer T et al. Monoclonal xenogeneic antibodies to murine cell surface antigens: identification of novel leukocyte differentiation antigens. Eur J Immunol 8:539-51 (1978). <PUBMED:81133> Milstein C et al. Monoclonal antibodies and cell surface antigens. Ciba Found Symp :251-76 (1979). <PUBMED:258170>

Additional Info

Detects the CD11b antigen (mW170 and 95kD) also known as Mac-1 antigen, found on mouse macrophages, their precursors and granulocytes. Species Crossreactivity: Crossreacts with Human, Mouse and Rabbit.

Alternative Names

Cell Surface Glycoprotein MAC1 Alpha Subunit, Complement Component Receptor 3 Alpha, CR3A, ITGAM, MAC1A, MO1A, Neutrophil Adherence Receptor