CD134 (CD134 Antigen, ACT35, ACT35 Antigen, Lymphoid Activation Antigen ACT35, OX40, OX40 Cell Surface Antigen, OX40 Homolog, OX40L Receptor, RP5-902P8.3, TAX Transcriptionally-activated Glycoprotein 1 Receptor, Tumor Necrosis Factor Receptor Superfamily

CD134, otherwise known as OX40, is a 43kD type I membrane protein and member of the tumor necrosis factor receptor superfamily, expressed predominantly by CD4+ activated T cells, and a key regulator of T cell-dependent immune responses.\n\nCD134 has been identified as the receptor for the OX40 ligand, CD252, expressed by activated B cells, acting as a co-stimulatory signal for the stimulation and secretion of immunoglobulins. CD134 has also been identified as a binding receptor for Feline Immunodeficiency Virus (FIV), acting along with CXC chemokine receptor 4 (CXCR4) to facilitate the entry of the virus into CD4+ primary target cells.\n\nApplications:\nSuitable for use in Flow Cytometry and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1ug/ml\nFlow Cytometry: 10ul labels 10e6 cells in 100ul\nOptimal dilutions to be determined by the researcher.\n\nHybridoma: \nNS0 myeloma cells with spleen cells from Balb/c mice.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C2515-16B

Size

250ug

Applications

FC, WB

Hosts

Mouse

Reactivities

Hum, Fel

Form

Supplied as a liquid in PBS, 0.09% sodium azide.

P Type

Mab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG1

References

1. Arch, R.H. and Thompson, C.B. (1998) 4-1BB and OX40 are members of a tumor necrosis factor (TNF)- nerve growth factor receptor subfamily that bind TNF receptor-associated factors and activate nuclear factor kappa B. Mol. Cell. Biol. 18: 558-565, 2. Stuber, E. and Strober, W. (1996) The T cell-B cell interaction via OX40-OX40L is necessary for the T-cell dependent humoral immune response. J. Exp. Med. 183: 979-989, 3. de Parseval, A. et al. (2004) Feline immunodeficiency virus targets activated CD4+ T cells by using CD134 as a binding receptor. P.N.A.S. 101: 13044-13049.

Additional Info

Recognizes feline CD134, otherwise known as OX40. Does not react with mouse. Species Crossreactivity: human (weakly).