CD158b (p58.2)

Applications:\nSuitable for use in Flow Cytometry and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilutions:\nFlow Cytometry: 1:10-1:50. Use 10ul to label 10e6 cells in 100ul.\nOptimal working dilutions to be determined by researcher.\n\nHybridoma: \nSpleen cells from immunized BALB/c mice were fused with cells of the mouse P3UI myeloma cell line.\n\nRecommended Negative Control: \nI1904-78D: Mouse IgG1 Negative Control\n\nRecommended Secondary Antibody:\nI1904-66A: IgG, F(ab

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

C2548-87B

Size

200ug

Applications

FC, IP

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide, 0.1% BSA.

P Type

Mab

Purity

Purified

Isotype

IgG1

References

1. Moretta, A. et al. (1990). A novel surface antigen expressed by a subset of human CD3-CD16+ natural killer cells. J. Exp. Med. 171: 695 - 714.2. Moretta, A. et al. (1993). p58 molecules as putative receptors for major histocompatibility complex (MHC) class-1 molecules in human natural killer (NK) cells. Anti-p58 antibodies reconstitute lysis of MHC class-1 protected cells in NK clones displaying different specificities. J. Exp. Med. 178: 597-604

Additional Info

Recognizes the human CD158b cell surface antigen, a 58kD glycoprotein expressed by a subset of NK cells (also known as p58.2 antigen).This antibody also recognizes a 50kD molecule, which is highly homologous to p58.2 in the extracellular domain, but has a shorter cytoplasmic tail. Both molecules are members of the newly described natural killer cell receptor family. CD158b functions as a receptor specific for HLA Class I molecules, including Cw3 and related HLA-C alleles. This antibody can restore the lysis by human NK clones of otherwise lysis protected targets expressing Cw3.