CD19 (B Lymphocyte Antigen CD19, B4, Differentiation Antigen CD19, Leu 12, Leu-12, MGC12802) (PE, Cy5)

CD19 is a member of the immunoglobulin superfamily with 556aa and two immunoglobulin-like C2-type domains. As a cell surface protein, CD19 is known to form a complex with CD21, CD81 and CD225 in the membrane of mature B cells. A major function of CD19 is to assemble with the antigen receptor of B lymphocytes so as to decrease the threshold for antigen receptor dependent stimulation thus enhancing the specificity and sensitivity of B cells towards antigens. CD19 is an important protein for B cell antigen receptor signaling and regulation and also acts as a specialized adaptor protein for the amplification of Src family needed for this purpose. Regulation of growth of B cells is a major function of CD19 and its expression is confined to only B lymphocytes and follicular dendritic cells of the hematopoietic system. Increased expression of CD19 induces the production of auto-antibody thus giving an insight to the regulatory role of CD19 in autoimmunity. Defects in CD19 are a cause of hypogammaglobulinemia.\n\nApplications:\nSuitable for use in Flow Cytometry. Other applications not tested.\n\nRecommended Dilution:\nFlow Cytometry: Neat-1:5; 10ul labels 10e6 cells in 100ul\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\n\nMay be stored at 4 degrees C before opening. DO NOT FREEZE! Stable at 4 degrees C as an undiluted liquid. Dilute only prior to immediate use. Stable for at least 6 months at 4 degrees C. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.

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SPECIFICATIONS

Catalog Number

C2269-82D12

Size

100Tests

Applications

FC

Hosts

Rat

Reactivities

Mouse

Form

Supplied as a liquid in PBS, sucrose, 0.09% sodium azide. Labeled with R-Phycoerythrin (PE)-Cy5.5.

P Type

Mab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG2a

References

1. Krop, I. et al. (1996) Self-renewal of B-1 lymphocytes is dependent on CD19 Eur. J. Immunol. 26: 238. 2. Vernooy, J. et al. (2002) Long-term intratracheal lipopolysaccharide exposure in mice results in chronic lung inflammation and persistent pathology. Am. J. Respir. Cell. Mol. Biol. 26: 152-159.

Additional Info

Recognizes mouse CD19 cell surface antigen, a 95kD glycoprotein expressed by B lymphocytes.

Alternative Names

CD19 Molecule