CD2 (E Rosette Receptor, T11, Lymphocyte Function Antigen 2, LFA-2, Sheep Erythrocyte Receptor) (Biotin)

CD2 which is also known as E-rosette receptor, T11 and lymphocyte-function antigen-2 (LFA-2) is expressed on T cells, thymocytes, and subset of natural killer cells. Human CD2 functions as the receptor for sheep erythrocytes, human CD58 (LFA-3), and CD15s (Sialyl Lewis X). p56lck, p59fyn, CD3eta and CD3epsilon are tyrosine-phosphorylated after CD2 stimulation. CD2 plays a role in T cell activation, T- or NK-cell-mediated cytolysis, apoptosis in activated peripheral T cells, and regulation of T cell anergy. Normal B lymphocytes, monocytes or granulocytes do not express surface CD2 antigen, neither do common ALL cells. The antigen has been characterized as the receptor for sheep erythrocytes and CD2 monoclonal antibodies that inhibit E rosette formation. CD2 interacts with lymphocyte function associated antigen (LFA3) and CD48/BCM1 to mediate adhesion between T cells and other cell types. CD2 is implicated in the triggerring of T cells, the cytoplasmic domain is implicated in the signaling function. It is useful for the identification of lymphomas and leukemias of T cell origin.\n\nApplications: \nSuitable for use in Flow Cytometry and Immunohistochemistry \nOther applications have not been tested. \n\nRecommended Dilutions: \nImmunohistochemistry: Frozen, Paraffin (see ref 2)\nFlow Cytometry: Neat-1:10. Use 10ul of the suggested working dilution to label 10e6 cells in 100ul.\nOptimal dilutions to be determined by the researcher. \n\nHybridoma: \nSpleen cells from immunized BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

C2253-07R

Size

100ug

Applications

FC, IHC

Hosts

Mouse

Reactivities

Mouse, Rat

Conjugates

Biotin

Form

Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide, 1% BSA. Labeled with Biotin.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG2a

References

1. Williams, A.F. et al. (1987). J. Exp. Med. 165: 368-380. 2. Whiteland, J.L. et al . (1995). Immunohistochemical Detection of T-Cell Subsets and other Leucocytes in Paraffin-embedded Rat and Mouse Tissues with Monoclonal Antibodies. J. Histochem. Cytochem. 43: 313-320. 3.Barclay A.N. Immunology 42:593-600 (1981).

Additional Info

Recognizes the rat CD2 cell surface antigen, a 50-54kD glycoprotein expressed by thymocytes and mature T cells.