CD2 (E Rosette Receptor, T11, Lymphocyte Function Antigen 2, LFA-2, Sheep Erythrocyte Receptor)

CD2 which is also known as E-rosette receptor, T11 and lymphocyte-function antigen-2 (LFA-2) is expressed on T cells, thymocytes, and subset of natural killer cells. Human CD2 functions as the receptor for sheep erythrocytes, human CD58 (LFA-3), and CD15s (Sialyl Lewis X). p56lck, p59fyn, CD3eta and CD3epsilon are tyrosine-phosphorylated\nafter CD2 stimulation. CD2 plays a role in T cell activation, T- or NK-cell-mediated cytolysis,\napoptosis in activated peripheral T cells, and regulation of T cell anergy. Normal B lymphocytes, monocytes or granulocytes do not express surface CD2 antigen, neither do common ALL cells. The antigen has been characterized as the receptor for sheep erythrocytes and CD2 monoclonal antibodies that inhibit E rosette formation. \n\nAntigen distribution: T cells (E+) 100% Thymocytes 95 + 3% Peripheral blood lymphocytes 82 + 5% NK cells (CD16+) 50 + 10% Granulocytes < 1% Monocytes < 1% B Cells (E-) < 1% \n\nRecommended Secondary Reagents: F(ab')2 rabbit anti-mouse IgG HRP \n\nFusion Partners: Spleen cells from immunized mice were fused with cells of the mouse p3-NS1-Ag4-1 myeloma cell line\n\nRecommended Positive Control Tissue: Human Tonsil\n\nRecommended Negative Controls: Mouse IgG1 Negative Control\n\nApplications:\nImmunohistochemistry: Paraffin. 1:40-1:80\nOptimal working dilutions to be determined by researcher.\n\nImmunohistochemistry: * This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. 1mM EDTA pH 8.0 is recommended for this purpose.

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