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CD23 (Blast 2, FC Epsilon RII, FC Receptor IgE Low Affinity II Alpha Polypeptide, FCER2A, IgE Binding Factor, IgE Receptor Lymphocyte, IGEBF, Low Affinity immunoglobulin, Ly42) Receptor Soluble Form, Ly42)

Cat no: C2274-02J

CD23 (Blast 2, FC Epsilon RII, FC Receptor IgE Low Affinity II Alpha Polypeptide, FCER2A, IgE Binding Factor, IgE Receptor Lymphocyte, IGEBF, Low Affinity immunoglobulin, Ly42) Receptor Soluble Form, Ly42)

CD23 is a 45kD protein, also known as low affinity IgE Fc receptor, FceRII, BLAST-2, Ly-42, or B6. It is a member of the Ig family, expressed on conventional B (but not B-1) cells and follicular dendritic cells. CD23 responds to high levels of IgE by downregulating IgE secretion. This antibody is useful for blocking IgE activity in vivo.\n\nApplications:\nSuitable for use in Flow Cytometry, Immunohistochemistry (frozen), and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nFlow Cytometry: 1:50-1:200; 10ul labels 10e6 cells in 100ul\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

C2274-02J

Size

250ug

Applications

FC, IHC, IP

Hosts

Rat

Reactivities

Mouse

Form

Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG2a

References

1. Rao, M. et al. (1987) Characterization of a monoclonal antibody directed against the murine B lymphocyte receptor for IgE. J. Immunol. 138:1845 - 1851, 2. Maeda, K. et al. (1992) Murine follicular dendritic cells and low affinity Fc receptors for IgE (Fc epsilonRII). J. Immunol. 148:2340

Additional Info

Recognizes mouse CD23.

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