Home  >  Products  >  CD23 (Blast 2, FC Epsilon RII, FC Receptor IgE Low Affinity II Alpha Polypeptide, FCER2A, IgE Binding Factor, IgE Receptor Lymphocyte, IGEBF, Low Affinity immunoglobulin, Ly42) Receptor Soluble Form, Ly42)
CD23 (Blast 2, FC Epsilon RII, FC Receptor IgE Low Affinity II Alpha Polypeptide, FCER2A, IgE Binding Factor, IgE Receptor Lymphocyte, IGEBF, Low Affinity immunoglobulin, Ly42) Receptor Soluble Form, Ly42)

CD23 (Blast 2, FC Epsilon RII, FC Receptor IgE Low Affinity II Alpha Polypeptide, FCER2A, IgE Binding Factor, IgE Receptor Lymphocyte, IGEBF, Low Affinity immunoglobulin, Ly42) Receptor Soluble Form, Ly42)

Cat no: C2274-02J1

Supplier: United States Biological
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CD23 is a 45kD protein, also known as low affinity IgE Fc receptor, FceRII, BLAST-2, Ly-42, or B6. It is a member of the Ig family, expressed on conventional B (but not B-1) cells and follicular dendritic cells. CD23 responds to high levels of IgE by downregulating IgE secretion. This antibody is useful for blocking IgE activity in vivo. Applications: Suitable for use in Flow Cytometry, Functional Assays, Immunohistochemistry (frozen), and Immunoprecipitation. Other applications not tested. Recommended Dilution: Flow Cytometry: 10ul labels 10e6 cells in 100ul Optimal dilutions to be determined by the researcher. Hybridoma: 1R983F rat myeloma cells with spleen cells from Lou/m rats. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: C2274-02J1
Reactivities: Mouse
Hosts: Rat
Applications: Flow Cytometry, Immunohistochemistry, Immunoprecipitation
Size: 500ug
Form: Supplied as a liquid in PBS, pH 7.4. No preservative added.
P type: Mab
Isotype: IgG2a
Purity: Purified by Protein G affinity chromatography.
References: 1. Rao, M. et al. (1987) Characterization of a monoclonal antibody directed against the murine B lymphocyte receptor for IgE. J. Immunol. 138:1845 - 1851, 2. Maeda, K. et al. (1992) Murine follicular dendritic cells and low affinity Fc receptors for IgE (Fc epsilonRII). J. Immunol. 148:2340
Additional info: Recognizes mouse CD23.

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