CD3 (T Cell Surface Glycoprotein CD3, T Cell Surface Antigen)

CD3 is a multi-subunit complex consisting of 4 different invariable membrane proteins, CD3E, CD3D, CD3G and CD3-omega. These CD3 proteins are expressed on the surface of T-cells and Thymocytes during thymocyte development. They are non-covalently associated with the TCR heterodimer and form the functional TCR-CD3 antigen receptor complex of the T-lymphocyte. CD3 expression is significant during Intrathymic T-lymphocyte maturation, TCR signaling and Thymocyte differentiation. Stimulation of TCR-CD3 complex results in activation of various protein tyrosine kinases including ZAP-70 affecting multiple cellular responses including T-cell proliferation, clonal expansion, induction of tolerance and apoptosis. Mutations in the sub-unit chains of CD3 have been associated with various immunodeficiency syndromes including SCID.\n\nApplications:\nSuitable for use in Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nImmunohistochemistry (formalin fixed paraffin embedded): 1:25-1:50\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nHuman tonsil\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

C2254-07A1

Size

500ul

Applications

IHC

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid, 0.05% sodium azide.

P Type

Mab

Purity

Supernatant

Isotype

IgG2a

References

1. Chetty et al. J Pathol 173: 303, 1994. 2. Clevers et al. Ann Review Immunol 6: 629, 1988. 3. Clevers et al. Eur J Immunol 18: 705, 1988. 4. Meuer et al. Immunol Today 10: 225, 1989. 5. Stanners et.al JBC 270 (51) 30635-30642 (December 22, 1995) 6. Kuhns et.al Immunity 24 (2) 133-139 (February 2006)

Additional Info

Recognizes human CD3.