CD3 (Cluster of Differentiation 3) is a 22kD protein that consists of five invariant polypeptide chains that associate to form three dimers: a heterodimer of gamma and epsilon chains, a heterodimer of delta and epsilon chains and a homodimer of two zeta chains or a heterodimer of zeta and eta chains. The zeta chain contains three consecutive copies of the same motif.
Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilutions:
Western Blot: 1:500-1:1000 detects a band at ~23kD.
ELISA: 1:5000-1:20,000
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.