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CD3 zeta (T-cell Surface Glycoprotein CD3 zeta Chain, T-cell Receptor T3 zeta Chain, CD247, CD3Z, T3Z, TCRZ)

Cat no: C2254-01U1

CD3 zeta (T-cell Surface Glycoprotein CD3 zeta Chain, T-cell Receptor T3 zeta Chain, CD247, CD3Z, T3Z, TCRZ)

CD3 (Cluster of Differentiation 3) is a 22kD protein that consists of five invariant polypeptide chains that associate to form three dimers: a heterodimer of gamma and epsilon chains, a heterodimer of delta and epsilon chains and a homodimer of two zeta chains or a heterodimer of zeta and eta chains. The zeta chain contains three consecutive copies of the same motif.\n\nApplications:\nSuitable for use in Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nImmunohistochemistry: 1:200 for 10min at RT\nThe staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10min followed by cooling at RT for 20min\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nJurkat cells, tonsil\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

C2254-01U1

Size

50ug

Applications

IHC, IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.05% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Additional Info

Recognizes human CD3 zeta. Species Crossreactivity: mouse and rat.

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