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CD3 zeta (T-cell Surface Glycoprotein CD3 zeta Chain, T-cell Receptor T3 zeta Chain, CD247, CD3Z, T3Z, TCRZ)

CD3 zeta (T-cell Surface Glycoprotein CD3 zeta Chain, T-cell Receptor T3 zeta Chain, CD247, CD3Z, T3Z, TCRZ)

Cat no: C2254-01U1

Supplier: United States Biological
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CD3 (Cluster of Differentiation 3) is a 22kD protein that consists of five invariant polypeptide chains that associate to form three dimers: a heterodimer of gamma and epsilon chains, a heterodimer of delta and epsilon chains and a homodimer of two zeta chains or a heterodimer of zeta and eta chains. The zeta chain contains three consecutive copies of the same motif. Applications: Suitable for use in Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry: 1:200 for 10min at RT The staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10min followed by cooling at RT for 20min Optimal dilutions to be determined by the researcher. Positive Control: Jurkat cells, tonsil Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: C2254-01U1
Reactivities: Human, Mouse, Rat
Hosts: Rabbit
Applications: Immunohistochemistry, Immunoprecipitation, Western Blot
Size: 50ug
Form: Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.05% sodium azide.
P type: Pab
Purity: Purified by immunoaffinity chromatography.
Additional info: Recognizes human CD3 zeta. Species Crossreactivity: mouse and rat.

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