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CD31 (CD31 Antigen, EndoCAM, GPIIA, Platelet Endothelial Cell Adhesion Molecule, Pecam 1, Pecam1, Pecam-1) (PE)

Cat no: C2383-03G5

CD31 (CD31 Antigen, EndoCAM, GPIIA, Platelet Endothelial Cell Adhesion Molecule, Pecam 1, Pecam1, Pecam-1) (PE)

PECAM-1 (CD31) is a type 1 transmembrane protein expressed primarily on endothelial cells, platelets and leukocytes.\n\nApplications:\nSuitable for use in Flow Cytometry. Other applications not tested.\n\nRecommended Dilutions:\nFlow Cytometry: 10ul labels 10e6 cells in 100ul\nOptimal dilutions to be determined by the researcher.\n\nHybridoma:\nSp2/0 myeloma cells with spleen cells from Balb/c mice. \n\nStorage and Stability:\nLyophilized powder may be stored at 4 degrees C. Reconstitute by adding sterile buffer or ddH2O. Aliquot and store at 4 degrees C. DO NOT FREEZE! Dilute only prior to immediate use. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C2383-03G5

Size

100Tests

Applications

FC

Hosts

Mouse

Reactivities

Rat

Form

Supplied as a lyophilized powder from PBS, pH 7.4, 1% BSA, 5% sucrose, 0.09% sodium azide. Labeled with R-Phycoerythrin (PE). Reconstitute with 1ml sterile ddH2O.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

1. Flaris, N.A. et al. (1993) Characterisation of microglia and macrophages in the central nervous system of the rat. GLIA 7: 34-40. 2. Williams, K.C. et al. (1996) PECAM-1 (CD31) expression in the central nervous system and its role in experimental allergic encephalomyelitis in the rat. J. Neurosci. Res. 45(6):747-57. 3. Stevenson, K.S. et al. (2009) Isolation, characterization, and differentiation of thy1.1-sorted pancreatic adult progenitor cell populations. Stem Cells Dev. 18: 1389-98. 4. Ott, I. et al. (2005) Endothelial-like cells expanded from CD34+ blood cells improve left ventricular function after experimental myocardial infarction. FASEB J. 19: 992-4. 5. Fujimoto, K.L. et al. (2007) An elastic, biodegradable cardiac patch induces contractile smooth muscle and improves cardiac remodeling and function in subacute myocardial infarction. J Am Coll Cardiol. 49: 2292-300. 6. Thebault, P. et al. (2010) The C-type lectin-like receptor CLEC-1, expressed by myeloid cells and endothelial cells, is up-regulated by immunoregulatory mediators and moderates T cell activation. J Immunol. 183: 3099-108. 7. Graham, M.J. et al. (1998) In vivo distribution and metabolism of a phosphorothioate oligonucleotide within rat liver after intravenous administration. J Pharmacol Exp Ther. 286: 447-58. 8. Haywood, L. et al. (2003) Inflammation and angiogenesis in osteoarthritis. Arthritis Rheum. 48: 2173-7. 9. Kielian, T. and Hickey, W.F. (2010) Proinflammatory cytokine, chemokine, and cellular adhesion molecule expression during the acute phase of experimental brain abscess development. Am J Pathol. 157: 647-58. 10. Lochhead, J.J. et al. (2010) Oxidative stress increases blood-brain barrier permeability and induces alterations in occludin during hypoxia-reoxygenation. J Cereb Blood Flow Metab. 30: 1625-36. 11. Nakao, A. et al. (2003) Carbon monoxide inhalation protects rat intestinal grafts from ischemia/reperfusion injury. Am J Pathol. 163: 1587-98. 12. Nakao, A. et al. (2011) Ex vivo carbon monoxide delivery inhibits intimal hyperplasia in arterialized vein grafts. Cardiovasc Res. 89: 457-63. 13. Ohnishi, T. et al. (2007) Comparison of endothelial cell proliferation in normal liver and adipose tissue in B6C3F1 mice, F344 rats, and humans. Toxicol Pathol. 35: 904-9. 14. Schilte, M.N. et al. (2009) Long-term intervention with heparins in a rat model of peritoneal dialysis. Perit Dial Int. 29: 26-35. 15. Seegers, H.C. et al. (2003) Enhancement of angiogenesis by endogenous substance P release and neurokinin-1 receptors during neurogenic inflammation. J Pharmacol Exp Ther. 306: 8-12. 16. Wilson, E. et al. (2007) An evaluation of the immunohistochemistry benefits of boric acid antigen retrieval on rat decalcified joint tissues. J Immunol Methods. 322: 137-42. 17. Willis, C.L. et al. (2010) Protein kinase C activation modulates reversible increase in cortical blood-brain barrier permeability and tight junction protein expression during hypoxia and posthypoxic reoxygenation. J Cereb Blood Flow Metab. 30: 1847-59. 18. Arkudas, A. et al. (2007) Fibrin gel-immobilized VEGF and bFGF efficiently stimulate angiogenesis in the AV loop model. Mol Med. 13: 480-7. 19. Salehi-Had, H. et al. (2011) Utilizing targeted gene therapy with nanoparticles binding alpha v beta 3 for imaging and treating choroidal neovascularization. PLoS One. 6: e18864.

Additional Info

Recognizes rat CD31. Shown to partially block the proliferative response of antigen-specific CD4+ T cells to antigen-presenting cells and relevant antigen. Does not react with human.

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SUPPLIER INFO

Applications

ELISA

Reactivities

Hum

More info

Applications

IF

Hosts

Mouse

More info

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, IHC, WB

Hosts

Mouse

More info

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum

More info

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

More info

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