CD3EAP (E474) pAb

CD3EAP, also known as ASE1, CAST or PAF49, is a 510 amino acid protein that localizes to the nucleus and belongs to the eukaryotic RPA34 RNA polymerase subunit family. Existing as two alternatively spliced isoforms, CD3EAP functions as a component of the Pol I complex, specifically exhibiting DNA-dependent RNA polymerase activity and effectively catalyzing the the conversion of a nucleoside triphosphate into a diphosphate, thereby transcribing DNA into RNA. Isoform 2 of CD3EAP may be a component of the T cell receptor (TCR) complex and is subject to tyrosine phosphorylation in response to TCR stimulation. Both isoforms of CD3EAP are subject to DNA damage-dependent phosphorylation, probably by ATM or ATR.

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SPECIFICATIONS

Catalog Number

BS3852

Size

100ug/100ul

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

liquid

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Antigen

Synthetic peptide, corresponding to amino acids 451-500 of Human CD3EAP.

Species

CD3EAP (E474) pAb detects endogenous levels of CD3EAP protein.

Dilutions

WB: 1:500~1:1000\r\nIHC: 1:50~1:200

Swiss Prot

O15446

Storage Temp

Store at 4 degrees C short term. Aliquot and store at -20 degrees C long term. Avoid freeze-thaw cycles.

Concentration

1ug/ul

Storage Buffer

1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.

Additional Info

For research use only, not for use in diagnostic procedure.

Molecular Weight

~ 55, 75 kDa

Alternative Names

DNA-directed RNA polymerase I subunit RPA34; A34.5; Antisense to ERCC-1 protein; ASE-1; CD3-epsilon-associated protein; CAST; CD3E-associated protein; RNA polymerase I-associated factor PAF49; ASE1; CAST; PAF49