CD4 (CD4 Receptor, CD4mut, T Cell Surface Antigen T4/Leu3, T Cell Surface Glycoprotein CD4)

Recommended Secondary Reagents: F(ab')2 rabbit anti-mouse IgG HRP, F(ab')2 rabbit anti-mouse IgG: RPE conjugate\n\nHybridoma: Spleen cells from immunized BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.\n\nRecommended Negative Controls: Mouse IgG1 Negative Control\n\nApplications:\nFlow Cytometry: 1:50-1:100\nImmunohistochemistry: (frozen)- 1:10-1:100\nOptimal working dilutions to be determined by researcher.\n\nFlow Cytometry: Use 10ul of the suggested working dilution to label 10e6 cells or 100ul whole blood.\nImmunohistochemistry: Recommended positive control: Human lymphatic tissue.\n\nStorage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be madeclosely approximates the binding spectrum of OKT4 and Leu-3 antibodies both of which also recognize 55 to 65% of human peripheral blood T-cells.\n\nRecommended Secondary Reagents: F(ab')2 rabbit anti-mouse IgG HRP, F(ab')2 rabbit anti-mouse IgG: RPE conjugate\n\nHybridoma: Spleen cells from immunized BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.\n\nRecommended Negative Controls: Mouse IgG1 Negative Control\n\nApplications:\nFlow Cytometry: 1:50-1:100\nImmunohistochemistry: (frozen)- 1:10-1:100\nOptimal working dilutions to be determined by researcher.\n\nFlow Cytometry: Use 10ul of the suggested working dilution to label 10e6 cells or 100ul whole blood.\nImmunohistochemistry: Recommended positive control: Human lymphatic tissue.\n\nStorage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C2255-27C

Size

200ug

Applications

FC, IHC

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

1. Voehringer, D. et al. (2002). Lack of proliferative capacity of human effector and memory T cells expressing killer cell lectin like receptor G1 (KLRG1). Blood. 100 (10): 3698 - 3702.

Additional Info

Recognizes the human CD4 cell surface antigen, a 55kD glycoprotein expressed by the helper-inducer subset of T lymphocytes and more weakly by monocytes.