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CD45 (Protein Tyrosine Phosphatase, Receptor Type, C, B220, CD45, CD45R, GP180, LCA, L-CA, Leukocyte Common Antigen, LY5, T200)

CD45 (Protein Tyrosine Phosphatase, Receptor Type, C, B220, CD45, CD45R, GP180, LCA, L-CA, Leukocyte Common Antigen, LY5, T200)

Cat no: C2400-10B

Supplier: United States Biological
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CD45 is a receptor-tyrosine phosphatase required for antigen-induced signaling in T-cells and B-cells; its substrates are unknown. Interestingly, it associates with several Src-family tyrosine kinases, which are inhibited by tyrosine phosphorylation in their c-termini, suggesting a mechanism for signaling by CD45. The LCA comprises of at least 5 isoforms ranging from 180-220kD which are produced as a result of alternative splicing of exons A, B or C. CBL 124 recognizes epitopes common to all of the isoforms. Antigen distribution: Thymocytes > 95% Granulocytes > 95% Monocytes > 95% B cells (CD20+) > 95% T cells (CD3+) > 95% NK cells (CD16+) > 95% Peripheral blood lymphocytes > 95% FUSION PARTNER: NS1 myeloma cell line Applications: Suitable for use in Immunofluorescence, Western Blot, Immunoprecipitation, Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:500 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: C2400-10B
Reactivities: Human
Hosts: Mouse
Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Size: 100ug
Form: Supplied as a liquid, 10mM sodium azide, 1 mg/mL BSA.
P type: Mab
Isotype: IgG2a
Purity: Purified by Protein A affinity chromatography.
References: 1. Justement, L. B., et al. (1994). Immunol. Today. 15:399-406. 2. Leucocyte Typing IV. (1989). Oxford University Press. 3. Leucocyte Typing III. (1987). Oxford University Press. 4. Dalchau, R., et al. (1980). Eur. J. Immunol. 10: 737.
Additional info: Recognizes the leucocyte common antigen (LCA) complex to be found on lymphocytes, monocytes, granulocytes, thymocytes and malignant T and B cells. No reactivity has been observed with primary or metastatic carcinoma cells. Plasma cells or myeloma cells may have weak expression or be negative for this antigen. Species Crossreactivity: human. Species sequence homology: mouse and rat.

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